Department of Microbiology, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.
Department of Biochemistry, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.
J Food Prot. 2014 Mar;77(3):435-43. doi: 10.4315/0362-028X.JFP-13-323.
The aim of this work was to characterize the essential oil (EO) of Eryngium foetidum (EfEO) and assess its activity toward Listeria monocytogenes in broth and during thermal inactivation of the pathogen in pineapple juice. In this respect, EfEO was chemically characterized, and its antilisteria potential in broth as a function of pH, cell load, and EfEO concentration was assessed through a central composite design. Furthermore, the inactivation kinetics of L. monocytogenes in the juice were assessed by combining EfEO and low pasteurization temperatures. A total of 81 compounds were identified from EfEO. The reduction of pH and cell load increased EO activity. The use of only 15 ppm of EfEO during pasteurization of pineapple juice at 60°C reduced the time required for a 4-log reduction in L. monocytogenes CFU/ml by 74.9% (i.e., from 8.5 to 2.1 min) compared with treatment without EfEO. It could be concluded that EfEO activity toward L. monocytogenes increases with the reduction of pH and that it can be used at sublethal concentrations in combination with low temperatures in pineapple juice pasteurization. This study demonstrates that EO-assisted pasteurization is a promising strategy for the reduction of thermal impact during juice production. EfEO is easily available and compatible with many juices and is thus promising for industrial application.
本工作旨在对 Foetid Eryngium（EfEO）精油进行特征描述，并评估其在肉汤中和热失活期间对单核细胞增生李斯特菌的活性。在这方面，对 EfEO 进行了化学特征描述，并通过中心复合设计评估了 EfEO 在 pH 值、细胞负荷和 EfEO 浓度作为功能的抗李斯特菌活性。此外，通过组合 EfEO 和低巴氏杀菌温度来评估 L. monocytogenes 在果汁中的失活动力学。从 EfEO 中鉴定出 81 种化合物。降低 pH 值和细胞负荷可提高精油的活性。与不使用 EfEO 相比，在 60°C 巴氏杀菌菠萝汁时，仅使用 15ppm 的 EfEO 可将单核细胞增生李斯特菌 CFU/ml 的 4 对数减少所需的时间从 8.5 分钟缩短至 2.1 分钟，降低了 74.9%。可以得出结论，EfEO 对 L. monocytogenes 的活性随着 pH 值的降低而增加，并且可以在亚致死浓度下与低温联合使用，用于菠萝汁巴氏杀菌。本研究表明，EO 辅助巴氏杀菌是减少果汁生产过程中热冲击的一种很有前途的策略。EfEO 易于获得，与许多果汁兼容，因此具有工业应用的前景。
