Kling H, Sawatzki G, Geis W
Abteilung Humangenetik, Universität Ulm, German Federal Republic.
Anal Biochem. 1988 Nov 1;174(2):589-92. doi: 10.1016/0003-2697(88)90060-7.
A rapid and highly sensitive silver stain for visualization of proteins on ultrathin isoelectric focusing gels is described. This procedure is based on the specific interaction of silver and bromide ions in the presence of proteins and appears to involve a precipitation reaction. The technique requires only two reaction solutions, a silver nitrate and a potassium bromide solution. Silver consumption is very low because the silver nitrate solution is reusable. This procedure is well established for proteins separated by isoelectrofocusing in ultrathin gels.
本文描述了一种用于在超薄等电聚焦凝胶上可视化蛋白质的快速且高度灵敏的银染方法。该方法基于在蛋白质存在下银离子和溴离子的特异性相互作用，似乎涉及沉淀反应。该技术仅需要两种反应溶液，即硝酸银溶液和溴化钾溶液。由于硝酸银溶液可重复使用，银的消耗量非常低。对于在超薄凝胶中通过等电聚焦分离的蛋白质，该方法已得到充分确立。
