Smith S L, Wilson P R, Collett M G, Heuer C, West D M, Stevenson M, Chambers J P
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
Vet Pathol. 2014 Sep;51(5):915-8. doi: 10.1177/0300985813516644. Epub 2014 Mar 27.
Sheep with Johne's disease develop epithelioid macrophage microgranulomas, specific to Mycobacterium avium subsp. paratuberculosis (Map) infection, in the terminal ileum, mesenteric lymph nodes, and organs distant to the alimentary tract such as the liver. The objectives of this study were to determine whether liver pathology was present in ewes affected by Map and whether liver cores provide adequate tissue for this potential diagnostic marker. One hundred and twenty-six adult, low body condition ewes were euthanized, necropsied, and underwent simulated liver biopsy. Ileal lesions typical of Map were found in 60 ewes. Hepatic epithelioid microgranulomas were observed in all ewes with Type 3b (n = 40) and 82% (n = 11) with Type 3c ileal lesions. None were found in ewes unaffected by Map or with Type 1, 2, or 3a ileal lesions. Liver biopsy core samples provided adequate tissue for histopathology with a sensitivity and specificity of 96% (95% confidence interval [CI], 0.87-0.99) and 100% (95% CI, 0.95-1), respectively for detection of types 3b and 3c ileal lesions.
患有副结核病的绵羊会在回肠末端、肠系膜淋巴结以及诸如肝脏等远离消化道的器官中形成上皮样巨噬细胞微肉芽肿，这是副结核分枝杆菌（Map）感染所特有的。本研究的目的是确定受Map感染的母羊是否存在肝脏病变，以及肝脏活检组织芯是否能为这种潜在的诊断标志物提供足够的组织。对126只成年、体况较差的母羊实施安乐死、进行尸检并进行模拟肝脏活检。在60只母羊中发现了典型的Map回肠病变。在所有患有3b型（n = 40）和82%（n = 11）患有3c型回肠病变的母羊中均观察到肝脏上皮样微肉芽肿。在未受Map感染或患有1型、2型或3a型回肠病变的母羊中未发现此类病变。肝脏活检组织芯样本为组织病理学提供了足够的组织，对于检测3b型和3c型回肠病变，其敏感性和特异性分别为96%（95%置信区间[CI]，0.87 - 0.99）和100%（95% CI，0.95 - 1）。
