Lehmann B, Taucher M, Kühne H, Scheuch D W
Medizinische Akademie Carl Gustav Carus Dresden.
Biomed Biochim Acta. 1988;47(4-5):363-71.
After separation of aceton and dextran sulfate activated human plasma by column chromatography on DEAE-cellulose three esterolytically and amidolytically active fractions, respectively, were obtained, which were assigned to the following species: plasma kallikrein (PK), PK.alpha-macroglobulin.HMW-Kininogen. Their percentage in the whole activity is variable. The proportion of free PK is low (0.11). For characterization of the products we studied inhibition by different polyvalent inhibitors. The Michaelis constant (Km) with p-toluene-sulfonyl-L-arginine methyl ester (TAME) were determined. For simulation of in vivo conditions dextran sulfate activated plasma was inactivated at 37 degrees C. The residual activity and the spontaneous activity in plasma from patients with shock are produced by different active protease inhibitor complexes.
通过在DEAE - 纤维素上进行柱色谱分离丙酮和硫酸葡聚糖活化的人血浆后，分别获得了三个具有酯解活性和酰胺解活性的组分，它们被归为以下种类：血浆激肽释放酶（PK）、PK.α - 巨球蛋白.HMW - 激肽原。它们在总活性中的百分比是可变的。游离PK的比例较低（0.11）。为了表征产物，我们研究了不同多价抑制剂的抑制作用。测定了对甲苯磺酰 - L - 精氨酸甲酯（TAME）的米氏常数（Km）。为了模拟体内条件，硫酸葡聚糖活化的血浆在37℃下失活。休克患者血浆中的残余活性和自发活性是由不同的活性蛋白酶抑制剂复合物产生的。
