Nishimura Yuri, Mabuchi Kaori, Taguchi Sayumi, Ikeda Saori, Aida Eri, Negishi Hiroko, Takamata Akira
Department of Environmental Health, Nara Women's University, Kitauoya Nishimachi, Nara, 630-8506, Japan.
J Physiol Sci. 2014 May;64(3):203-11. doi: 10.1007/s12576-014-0312-0. Epub 2014 Mar 28.
In order to elucidate the involvement of melanin-concentrating hormone (MCH) and orexin-A (ORX-A) neurons of the perifornical/lateral hypothalamic areas (PF/LH) in the regulation of food intake induced by acutely reduced glucose availability, we examined the food intake response and c-Fos expression in the MCH and ORX-A neurons in the PF/LH during 2-deoxy-D-glucose (2DG)-induced glucoprivation (400 mg/kg; i.v.) and systemic insulin-induced hypoglycemia (5 U/kg; s.c.) in male Wistar rats. The administration of both 2DG and insulin stimulated food intake and induced c-Fos expression in the ORX-A neurons corresponding to food intake, but not in the MCH neurons. These data indicate that ORX-A neurons, but not MCH neurons, play a role in the short-term regulation of food intake, and that the input signals for the neurons containing MCH and ORX-A are different, and these neurons play different roles in the regulation of feeding behavior.
为了阐明穹窿周/下丘脑外侧区（PF/LH）的促黑素细胞激素（MCH）和食欲素A（ORX-A）神经元在急性葡萄糖供应减少诱导的食物摄入调节中的作用，我们检测了雄性Wistar大鼠在2-脱氧-D-葡萄糖（2DG）诱导的糖剥夺（400mg/kg；静脉注射）和全身胰岛素诱导的低血糖（5U/kg；皮下注射）过程中，PF/LH中MCH和ORX-A神经元的食物摄入反应及c-Fos表达。2DG和胰岛素的给药均刺激了食物摄入，并在与食物摄入相应的ORX-A神经元中诱导了c-Fos表达，但在MCH神经元中未诱导。这些数据表明，ORX-A神经元而非MCH神经元在食物摄入的短期调节中起作用，并且含有MCH和ORX-A的神经元的输入信号不同，这些神经元在进食行为调节中发挥不同作用。
