Czerniak B, Chen R, Tuziak T, Markiewski M, Kram A, Gorczyca W, Deitch D, Herz F, Koss L G
Department of Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York 10467.
Cancer. 1989 May 15;63(10):2008-13. doi: 10.1002/1097-0142(19890515)63:10<2008::aid-cncr2820631024>3.0.co;2-d.
The oncogenes most frequently detected in human tumors belong to the ras gene family (Ha-ras, Ki-ras, and N-ras). These genes encode a group of closely related 21,000 dalton proteins termed p21. An immunohistochemical study of ras p21 expression was carried out on paraffin sections of 54 human breast carcinomas using monoclonal antibodies to p21. The control group consisted of ten cases of benign fibrocystic disease. The p21 expression was significantly higher in cancer cells than in epithelial cells of control specimens. No correlations, however, were observed between oncogene product expression and tumor size, histologic type, or grade. As a group, tumors with axillary lymph node metastases expressed higher levels of ras p21 than nonmetastasizing tumors. However, because of the significant overlap in individual p21 values, it is unlikely that the immunohistochemical assay for p21 could be used to predict the behavior of mammary carcinomas.
在人类肿瘤中最常检测到的癌基因属于ras基因家族（Ha-ras、Ki-ras和N-ras）。这些基因编码一组紧密相关的21000道尔顿的蛋白质，称为p21。使用针对p21的单克隆抗体，对54例人类乳腺癌的石蜡切片进行了ras p21表达的免疫组织化学研究。对照组由10例良性纤维囊性疾病病例组成。癌细胞中的p21表达明显高于对照标本的上皮细胞。然而，未观察到癌基因产物表达与肿瘤大小、组织学类型或分级之间的相关性。总体而言，有腋窝淋巴结转移的肿瘤比无转移的肿瘤表达更高水平的ras p21。然而，由于个体p21值存在显著重叠，p21的免疫组织化学检测不太可能用于预测乳腺癌的行为。
