Research Center for Clinical Pharmacy, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China.
J Anal Methods Chem. 2014;2014:629343. doi: 10.1155/2014/629343. Epub 2014 Feb 11.
A highly sensitive, simple, and rapid liquid chromatography tandem mass spectrometry method to simultaneously determine blonanserin and blonanserin C in human plasma with AD-5332 as internal standard (IS) was established. A simple direct protein precipitation method was used for the sample pretreatment, and chromatographic separation was performed on a Waters XBridge C8 (4.6 × 150 mm, 3.5 μ m) column. The mobile phase consists of a mixture of 10 mM ammonium formate and 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B). To quantify blonanserin, blonanserin C, and IS, multiple reaction monitoring (MRM) was performed in positive ESI mode. The calibration curve was linear in the concentration range of 0.012-5.78 ng·mL(-1) for blonanserin and 0.023-11.57 ng·mL(-1) for blonanserin C (r (2) > 0.9990). The intra- and interday precision of three quality control (QC) levels in plasma were less than 7.5%. Finally, the current simple, sensitive, and accurate LC-MS/MS method was successfully applied to investigate the pharmacokinetics of blonanserin and blonanserin C in healthy Chinese volunteers.
建立了一种灵敏、简便、快速的液相色谱-串联质谱法，同时测定人血浆中的博兰色林及其代谢物博兰色林 C。采用 AD-5332 作为内标（IS），采用简单的直接蛋白沉淀法进行样品预处理，在 Waters XBridge C8（4.6×150mm，3.5μm）柱上进行色谱分离。流动相由 10mM 甲酸铵和 0.1%甲酸的水溶液（A）和 0.1%甲酸的甲醇溶液（B）组成。为了定量测定博兰色林、博兰色林 C 和 IS，采用正离子电喷雾模式进行多重反应监测（MRM）。博兰色林的校准曲线在 0.012-5.78ng·mL(-1)浓度范围内呈线性（r (2) > 0.9990），博兰色林 C 的校准曲线在 0.023-11.57ng·mL(-1)浓度范围内呈线性（r (2) > 0.9990）。三种质控（QC）水平的日内和日间精密度均小于 7.5%。最后，该简单、灵敏、准确的 LC-MS/MS 方法成功应用于研究健康中国志愿者中博兰色林及其代谢物博兰色林 C 的药代动力学。
