Cátedra de Química Agrícola, E.T.S.I. Agrónomos, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, Spain.
Cátedra de Química Agrícola, E.T.S.I. Agrónomos, Universidad de Castilla-La Mancha, Campus Universitario, 02071 Albacete, Spain.
Food Chem. 2014 Aug 15;157:518-23. doi: 10.1016/j.foodchem.2014.02.088. Epub 2014 Feb 28.
The aim of this work is to propose an HPLC method for analysing major steviol glycosides as well as to optimise the extraction and clarification conditions for obtaining these compounds. Toward this aim, standards of stevioside and rebaudioside A with purities ⩾99.0%, commercial samples from different companies and Stevia rebaudiana Bertoni leaves from Paraguay supplied by Insobol, S.L., were used. The analytical method proposed is adequate in terms of selectivity, sensitivity and accuracy. Optimum extraction conditions and adequate clarification conditions have been set. Moreover, this methodology is safe and eco-friendly, as we use only water for extraction and do not use solid-phase extraction, which requires solvents that are banned in the food industry to condition the cartridge and elute the steviol glycosides. In addition, this methodology consumes little time as leaves are not ground and the filtration is faster, and the peak resolution is better as we used an HPLC method with gradient elution.
本工作旨在提出一种 HPLC 方法来分析主要甜菊糖苷，并优化提取和澄清条件以获得这些化合物。为此，使用了纯度 ⩾99.0%的甜菊苷和瑞鲍迪苷 A 标准品、来自不同公司的商品样品以及由 Insobol，S.L. 提供的来自巴拉圭的甜叶菊（Stevia rebaudiana Bertoni）叶。所提出的分析方法在选择性、灵敏度和准确性方面是适当的。已经设定了最佳提取条件和适当的澄清条件。此外，由于我们仅使用水进行提取，并且不使用固相萃取，因此该方法是安全且环保的，固相萃取需要在食品工业中禁用的溶剂来调节柱并洗脱甜菊糖苷。此外，由于叶子不需要研磨，过滤更快，并且使用梯度洗脱的 HPLC 方法可以获得更好的峰分辨率，因此该方法耗时较少。
