Cebrián-Tarancón Cristina, Oliva José, Cámara Miguel Ángel, Alonso Gonzalo L, Salinas M Rosario
Cátedra de Química Agrícola, E.T.S.I. Agrónomos y Montes, Departamento de Ciencia y Tecnología Agroforestal y Genética, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, Spain.
Departamento de Química Agrícola, Geología y Edafología, Facultad de Química, Universidad de Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain.
Foods. 2021 Jan 19;10(1):191. doi: 10.3390/foods10010191.
Nowadays, the techniques for the analysis of glycosidic precursors in grapes involve changes in the glycoside structure or it is necessary the use of very expensive analytical techniques. In this study, we describe for the first time an approach to analyse intact glycosidic aroma precursors in grapes by high-performance liquid chromatography with a diode array detector (HPLC-DAD), a simple and cheap analytical technique that could be used in wineries. Briefly, the skin of Muscat of Alexandria grapes was extracted using a microwave and purified using solid-phase extraction combining Oasis MCX and LiChrolut EN cartridges. In total, 20 compounds were selected by HPLC-DAD at 195 nm and taking as a reference the spectrum of phenyl β-D-glucopyranoside, whose DAD spectrum showed a first shoulder from 190 to 230 nm and a second around 200-360 nm. After that, these glycosidic compounds were identified by High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-qTOF-MS). Disaccharides hexose pentose were the most abundant group observed with respect to the sugars and monoterpendiols the main aglycones found.
如今，用于分析葡萄中糖苷前体的技术涉及糖苷结构的变化，或者需要使用非常昂贵的分析技术。在本研究中，我们首次描述了一种通过高效液相色谱-二极管阵列检测器（HPLC-DAD）分析葡萄中完整糖苷香气前体的方法，这是一种可在酿酒厂使用的简单且廉价的分析技术。简要地说，亚历山大麝香葡萄的果皮采用微波提取，并使用结合了Oasis MCX和LiChrolut EN柱的固相萃取进行纯化。通过HPLC-DAD在195 nm波长下总共筛选出20种化合物，并以苯基β-D-吡喃葡萄糖苷的光谱作为参考，其DAD光谱在190至230 nm处有第一个峰肩，在200 - 360 nm左右有第二个峰肩。之后，这些糖苷化合物通过高效液相色谱-四极杆飞行时间质谱（HPLC-qTOF-MS）进行鉴定。就糖类而言，二糖己糖戊糖是观察到的最丰富的类别，单萜二醇是发现的主要糖苷配基。
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