Tian Wen-Liang, He Fei, Fu Xue, Lin Jun-Tang, Tang Ping, Huang Yu-Min, Guo Rong, Sun Ling
Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province 450052, China.
Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province 450052, China.
Gene. 2014 Jun 1;542(2):122-8. doi: 10.1016/j.gene.2014.03.046. Epub 2014 Mar 25.
This study investigated the expression of heat shock protein 90 alpha (Hsp90α) in acute leukemia cells. The expression of Hsp90α was investigated in leukemia cell lines and human bone marrow mononuclear cells derived from acute leukemia patients and from healthy individuals using polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay. Compared with cells from healthy individuals, the expression of Hsp90α in the untreated patients was higher. Similarly high levels were observed in remission patients. Significantly higher expression levels were observed in all the tested cell lines, and in cells from refractory and relapsed patients. No obvious relationship was observed between the occurrence of graft versus host disease and the expression of Hsp90α. The untreated patients showing higher expression levels of Hsp90α had lower complete remission rates. During remission of untreated patients, the expression of Hsp90α decreased and reached the lowest level after transplantation, but the expression increased again before relapse. Hsp90α was highly expressed in leukemia cells. The expression level of Hsp90α was associated with leukemia prognosis. However, no obvious relationship was observed between the occurrence of graft versus host disease and the expression of Hsp90α.
本研究调查了热休克蛋白90α（Hsp90α）在急性白血病细胞中的表达情况。使用聚合酶链反应、蛋白质免疫印迹法和酶联免疫吸附测定法，对白血病细胞系以及源自急性白血病患者和健康个体的人骨髓单个核细胞中Hsp90α的表达进行了研究。与健康个体的细胞相比，未经治疗的患者中Hsp90α的表达更高。在缓解期患者中也观察到了类似的高水平表达。在所有测试的细胞系以及难治性和复发性患者的细胞中，均观察到明显更高的表达水平。未观察到移植物抗宿主病的发生与Hsp90α表达之间存在明显关系。Hsp90α表达水平较高的未经治疗患者的完全缓解率较低。在未经治疗患者的缓解期，Hsp90α的表达下降，在移植后达到最低水平，但在复发前再次升高。Hsp90α在白血病细胞中高表达。Hsp90α的表达水平与白血病预后相关。然而，未观察到移植物抗宿主病的发生与Hsp90α表达之间存在明显关系。
