Mazza M M, Retegui L A
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
Mol Immunol. 1989 Mar;26(3):231-40. doi: 10.1016/0161-5890(89)90076-x.
Murine monoclonal antibodies (MAb) have been used as tools to probe the antigenic topography of human growth hormone (hGH). Mapping experiments were carried out by testing the ability of paired MAb to bind simultaneously or separately to 125I-hGH. A putative three-dimensional model of the relative distribution of 20 hGH epitopes indicated that they covered the entire molecular surface, showing the following essential characteristics. A domain of unique hGH specificity representing approximately 20% of the whole area was detected, as well as the presence of a discontinuous band of immunological identity between hGH and human placental lactogen (hPL) occupying 30% of the molecular surface. The rest of the surface (about 50%) displayed only partial cross-reactivity with hPL. Three restricted antigenic areas were also recognized. One of them appeared to correlate with a conformational change induced by the adsorption of the protein to plastic surfaces and the other two showed cross-reactivity with human prolactin and heterologous GH, respectively.
鼠单克隆抗体（MAb）已被用作探测人生长激素（hGH）抗原拓扑结构的工具。通过测试配对单克隆抗体同时或分别与¹²⁵I-hGH结合的能力进行了定位实验。一个关于20个hGH表位相对分布的假定三维模型表明，它们覆盖了整个分子表面，显示出以下基本特征。检测到一个代表整个区域约20%的具有独特hGH特异性的结构域，以及hGH与人胎盘催乳素（hPL）之间占分子表面30%的一条不连续免疫同一性带。表面的其余部分（约50%）仅与hPL表现出部分交叉反应性。还识别出三个受限抗原区域。其中一个似乎与蛋白质吸附到塑料表面诱导的构象变化相关，另外两个分别与人催乳素和异种生长激素表现出交叉反应性。
