采用 EPA 方法 1611 和方法 1609 PCR 试剂对美国中西部河流样本中肠球菌定量聚合酶链反应分析结果的比较。
Comparison of Enterococcus quantitative polymerase chain reaction analysis results from Midwest U.S. river samples using EPA Method 1611 and Method 1609 PCR reagents.
机构信息
U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA.
U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA.
出版信息
J Microbiol Methods. 2014 Jun;101:9-17. doi: 10.1016/j.mimet.2014.03.004. Epub 2014 Mar 26.
Enterococci target sequence density estimates from analyses of diluted river water DNA extracts by EPA Methods 1611 and 1609 and estimates with lower detection limits from undiluted DNA extracts by Method 1609 were indistinguishable. These methods should be equally suitable for comparison with U.S. EPA 2012 Recreational Water Quality Criteria values.
通过 EPA 方法 1611 和 1609 对稀释河水 DNA 提取物进行分析，以及通过未经稀释的 DNA 提取物采用方法 1609 得到的更低检测限的 Enterococci 目标序列密度估算值之间没有区别。这些方法应同样适用于与美国环保署 2012 年娱乐用水水质标准值进行比较。
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