Newton S M, Jacob C O, Stocker B A
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305.
Science. 1989 Apr 7;244(4900):70-2. doi: 10.1126/science.2468182.
Bacterial flagella are potent immunogens and aromatic-dependent (aro) Salmonella as live vaccines evoke humoral and cellular immune responses. Such strains expressing epitopes of protective antigens as inserts in flagellin would provide a novel way to vaccinate against diseases caused by unrelated pathogens. A synthetic oligonucleotide specifying an epitope of cholera toxin subunit B was inserted in a Salmonella flagellin gene. The chimeric flagellin functioned normally and the epitope was expressed at the flagellar surface. Parenteral administration to mice of an aro A flagellin-negative strain of S. dublin expressing the chimeric flagellin gene evoked antibody to cholera toxin.
细菌鞭毛是强效免疫原，而作为活疫苗的芳香族依赖型（aro）沙门氏菌可引发体液免疫和细胞免疫反应。此类表达保护性抗原表位并插入鞭毛蛋白的菌株可为预防由不相关病原体引起的疾病提供一种新的疫苗接种方式。将一段指定霍乱毒素B亚单位表位的合成寡核苷酸插入沙门氏菌鞭毛蛋白基因中。嵌合鞭毛蛋白功能正常，且该表位在鞭毛表面表达。对小鼠进行肠胃外接种表达嵌合鞭毛蛋白基因的aro A鞭毛蛋白阴性都柏林沙门氏菌菌株，可诱发针对霍乱毒素的抗体。
