Golden G T, Reyes P F, Fariello R G
Research Service, Veterans Administration Medical Center, Coatesville, PA 19320.
Brain Res Bull. 1989 Feb;22(2):461-8. doi: 10.1016/0361-9230(89)90073-7.
Donor tectal tissue from fetal rats was transplanted to the tectal region of intact host newborn rats. Histological and ultrastructural characteristics of mature tectal grafts were examined with special emphasis placed on glial elements and the interface between transplant and host brain. Most transplants survived, proliferated, and differentiated to mature neurons and glial cells. Mature transplants were found to contain small and medium sized neurons which formed clusters along the transplant border. Neurites traversed the interface between transplant and host brain. There was an absence of phagocytosis, inflammatory reactions and chromatolytic neurons throughout the transplant. Classical staining techniques and GFAP immunoreactive stains revealed only mild focal astrogliosis confined mostly to the subpial and perivascular regions of the transplant-host interface. Ultrastructural examination of the transplant revealed numerous axodendritic synapses with aggregates of synaptic vesicles and well formed postsynaptic membranes. The absence of a leptomeningeal membrane at several points along the transplant-host interface may allow neurites from both sides to establish functional synaptic contacts. Single unit recording combined with anterograde WGA-HRP neuronal tract tracing was used to determine the presence and extent of host retinal innervation of tectal transplants.
将胎鼠的供体顶盖组织移植到完整宿主新生大鼠的顶盖区域。检查成熟顶盖移植物的组织学和超微结构特征，特别关注神经胶质成分以及移植物与宿主脑之间的界面。大多数移植物存活、增殖并分化为成熟的神经元和神经胶质细胞。发现成熟移植物含有中小型神经元，这些神经元沿移植物边界形成簇。神经突穿过移植物与宿主脑之间的界面。整个移植物中均未出现吞噬作用、炎症反应和染色质溶解神经元。经典染色技术和GFAP免疫反应性染色显示，仅在移植物-宿主界面的软膜下和血管周围区域存在轻度局灶性星形胶质细胞增生。对移植物的超微结构检查显示，有大量轴突-树突突触，伴有突触小泡聚集和形成良好的突触后膜。沿移植物-宿主界面的几个点处缺乏软脑膜，这可能使两侧的神经突建立功能性突触联系。采用单单位记录结合顺行WGA-HRP神经元束追踪技术来确定宿主视网膜对顶盖移植物的支配情况及范围。
