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从猪（野猪）血清中纯化酸稳定的胰岛素样生长因子结合蛋白。

Purification of the serum acid-stable insulin-like growth factor binding protein from the pig (Sus scrofa).

作者信息

Walton P E, Baxter R C, Burleigh B D, Etherton T D

机构信息

Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.

出版信息

Comp Biochem Physiol B. 1989;92(3):561-7. doi: 10.1016/0305-0491(89)90132-6.

DOI:10.1016/0305-0491(89)90132-6
PMID:2468442
Abstract
  1. An acid-stable IGF binding protein was isolated and purified from porcine serum. 2. The protein comprised two major species with Mrs of 45 and 41 kDa determined using SDS-PAGE under reducing conditions. 3. The IGFBP preparation specifically bound both IGF-I and II. 4. Four distinct protein bands (Mrs of 23, 45, 50 and 75 kDa) in the porcine IGFBP preparation specifically bound radiolabelled IGF-I. 5. The porcine IGFBP exhibited sequence homology with IGFBPs from human plasma and rat serum. 6. This is the first report of the purification and characterization of the acid-stable IGFBP from porcine serum.
摘要
  1. 从猪血清中分离并纯化出一种酸稳定的胰岛素样生长因子结合蛋白。2. 该蛋白包含两个主要条带，在还原条件下用SDS-PAGE测定其分子量分别为45 kDa和41 kDa。3. 该胰岛素样生长因子结合蛋白制剂能特异性结合胰岛素样生长因子-I和-II。4. 猪胰岛素样生长因子结合蛋白制剂中的四条不同蛋白条带（分子量分别为23 kDa、45 kDa、50 kDa和75 kDa）能特异性结合放射性标记的胰岛素样生长因子-I。5. 猪胰岛素样生长因子结合蛋白与人血浆和大鼠血清中的胰岛素样生长因子结合蛋白具有序列同源性。6. 这是关于从猪血清中纯化和鉴定酸稳定的胰岛素样生长因子结合蛋白的首次报道。

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Comp Biochem Physiol B. 1989;92(3):561-7. doi: 10.1016/0305-0491(89)90132-6.
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引用本文的文献

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Postsynthetic regulation of insulin-like growth factor-binding protein-3 by MCF-7 human breast cancer cells in culture.培养的MCF-7人乳腺癌细胞对胰岛素样生长因子结合蛋白-3的合成后调控
Breast Cancer Res Treat. 1996;39(2):187-96. doi: 10.1007/BF01806185.
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Glycosaminoglycans inhibit formation of the 140 kDa insulin-like growth factor-binding protein complex.糖胺聚糖抑制140 kDa胰岛素样生长因子结合蛋白复合物的形成。
Biochem J. 1990 Nov 1;271(3):773-7. doi: 10.1042/bj2710773.