Ferris D K, Willette-Brown J, Martensen T, Farrar W L
Program Resources Inc., Biological Carcinogenesis & Development Program, ESP, Frederick Cancer Research Facility, MD 21701-1013.
FEBS Lett. 1989 Mar 27;246(1-2):153-8. doi: 10.1016/0014-5793(89)80273-x.
FDC-P1 is a murine myeloid cell line that requires interleukin 3 (IL3) for survival and proliferation. While the biological effects of IL3 have been well described, the biochemical mechanisms of IL3 actions have only recently been examined. We have investigated whether IL3 or PMA stimulates phosphorylation of proteins on tyrosine as well as on serine/threonine residues as previously described [(1986) Blood 68, 906-913; (1987) Biochem. J. 244, 683-691]. Here we report that both IL3 and PMA stimulate the tyrosine phosphorylation of at least two proteins: pp70 and pp50 in FDC-P1 cells.
FDC-P1是一种鼠类骨髓细胞系，其存活和增殖需要白细胞介素3（IL3）。虽然IL3的生物学效应已得到充分描述，但IL3作用的生化机制直到最近才被研究。我们已经研究了IL3或佛波酯（PMA）是否如先前所述[(1986)《血液》68, 906 - 913；(1987)《生物化学杂志》244, 683 - 691]刺激蛋白质酪氨酸以及丝氨酸/苏氨酸残基的磷酸化。在此我们报告，IL3和PMA都能刺激FDC-P1细胞中至少两种蛋白质的酪氨酸磷酸化：pp70和pp50。
