Azhdari-Zarmehri Hassan, Semnanian Saeed, Fathollahi Yaghoub
Department of Basic Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran; Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Pharmacol Biochem Behav. 2014 Jul;122:286-90. doi: 10.1016/j.pbb.2014.03.017. Epub 2014 Mar 29.
Orexins are produced from neurons which are restricted to a few regions of the lateral hypothalamus (LH), where they are important in pain modulation. The orexin receptors and orexinergic projections are localized in regions previously shown to play a role in pain modulation such as rostral ventromedial medulla (RVM). The effect of orexin-A (ORXA) microinjection into the RVM on nociceptive behaviors was examined using the formalin test. Microinjection of ORXA into the RVM, but not adjacent reticularis gigantocellularis (Gi) nucleus, decreased formalin induced nociceptive behaviors. Pretreatment with a selective OX1R antagonist, SB-334867 inhibited the antinociception produced by ORXA, while the administration of SB-334867 alone had no effect. These data demonstrate that ORXA-induced antinociception in the formalin test is mediated in part through orexin1 receptors in the RVM.
食欲肽由局限于下丘脑外侧区(LH)少数区域的神经元产生,在这些区域食欲肽对疼痛调节很重要。食欲肽受体和食欲肽能投射定位于先前已证明在疼痛调节中起作用的区域,如延髓头端腹内侧区(RVM)。使用福尔马林试验研究了向RVM微量注射食欲肽A(ORXA)对伤害性行为的影响。向RVM而非相邻的巨细胞网状核(Gi)微量注射ORXA,可减少福尔马林诱导的伤害性行为。用选择性OX1R拮抗剂SB-334867预处理可抑制ORXA产生的抗伤害作用,而单独给予SB-334867则无作用。这些数据表明,在福尔马林试验中,ORXA诱导的抗伤害作用部分是通过RVM中的食欲肽1受体介导的。