向大鼠延髓头端腹内侧注射食欲素受体1拮抗剂可增强游泳应激诱导的抗伤害感受作用。
Administration of orexin receptor 1 antagonist into the rostral ventromedial medulla increased swim stress-induced antinociception in rat.
作者信息
Soliemani Neda, Moslem Alireza, Shamsizadeh Ali, Azhdari-Zarmehri Hassan
机构信息
Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
出版信息
Iran J Basic Med Sci. 2016 May;19(5):542-9.
OBJECTIVES
Intracerebroventricular injection of orexin-A (hypocretin-1) antagonist has been shown to inhibit stress-induced analgesia. However the locations of central sites that may mediate these effects have not been totally demonstrated. This study was performed to investigate the role of rostral ventromedial medulla (RVM) orexin receptor 1 in stress-induced analgesia (SIA).
MATERIALS AND METHODS
Forced swim stress in water was employed to adult male rats (200-250 g). Nociceptive responses were measured by formalin test (50 µl injection of formalin 2% subcutaneously into hind paw) and, pain related behaviors were monitored for 90 min following intra-microinjection of SB-334867 (orexin receptor 1 antagonist) into RVM.
RESULTS
Exposure to swimming stress test after administration of SB-334867 into RVM significantly reduces the formalin-induced nociceptive behaviors in phase1, interphase, and phase 2 in rats.
CONCLUSION
The result demonstrated the involvement of OXR1 in antinociceptive behaviors induced by swim stress in RVM.
目的
脑室内注射食欲素 - A（下丘脑泌素 - 1）拮抗剂已被证明可抑制应激诱导的镇痛作用。然而，可能介导这些效应的中枢位点尚未完全明确。本研究旨在探讨延髓头端腹内侧区（RVM）食欲素受体1在应激诱导镇痛（SIA）中的作用。
材料与方法
对成年雄性大鼠（200 - 250克）采用水浸强迫游泳应激。通过福尔马林试验（将50微升2%福尔马林皮下注射到后爪）测量伤害性反应，并在向RVM微量注射SB - 334867（食欲素受体1拮抗剂）后监测90分钟的疼痛相关行为。
结果
在向RVM注射SB - 334867后进行游泳应激试验，可显著降低大鼠在福尔马林诱导的第1期、间期和第2期的伤害性行为。
结论
结果表明OXR1参与了RVM中游泳应激诱导的抗伤害性行为。
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