AgResearch Ltd, Lincoln Science Centre, Private Bag 4749, Christchurch, New Zealand.
AgResearch Ltd, Lincoln Science Centre, Private Bag 4749, Christchurch, New Zealand.
Environ Pollut. 2014 Jul;190:1-9. doi: 10.1016/j.envpol.2014.03.008. Epub 2014 Mar 29.
Pollution induced community tolerance (PICT) to Cu(2+), and co-tolerance to nanoparticulate Cu, ionic silver (Ag(+)), and vancomycin were measured in field soils treated with Cu(2+) 15 years previously. EC50 values were determined using substrate induced respiration and correlations made against soil physicochemical properties, microbial community structure, physiological status (qCO2; metabolic quotient), and abundances of genes associated with metal and antibiotic resistance. Previous level of exposure to copper was directly (P < 0.05) associated with tolerance to addition of new Cu(2+), and also of nanoparticle Cu. However, Cu-exposed communities had no co-tolerance to Ag(+) and had increased susceptibly to vancomycin. Increased tolerance to both Cu correlated (P < 0.05) with increased metabolic quotient, potentially indicating that the community directed more energy towards cellular maintenance rather than biomass production. Neither bacterial or fungal community composition nor changes in the abundance of genes involved with metal resistance were related to PICT or co-tolerance mechanisms.
15 年前用 Cu(2+)处理过的田间土壤中，测定了污染诱导的群落耐受（PICT）对 Cu(2+)、纳米 Cu、离子银（Ag(+)）和万古霉素的共耐受能力。使用基质诱导呼吸法测定 EC50 值，并与土壤物理化学性质、微生物群落结构、生理状态（qCO2；代谢商）以及与金属和抗生素抗性相关的基因丰度进行相关性分析。先前接触铜的水平与添加新的 Cu(2+)以及纳米 Cu 的耐受能力直接相关（P < 0.05）。然而，暴露于铜的群落对 Ag(+)没有共耐受能力，对万古霉素的敏感性增加。对 Cu 的耐受性增加（P < 0.05）与代谢商增加相关，这可能表明群落将更多的能量用于细胞维持而不是生物量生产。细菌或真菌群落组成以及与金属抗性相关的基因丰度的变化均与 PICT 或共耐受机制无关。
