基于金属纳米粒子催化形成的新型 Hg2+ 电化学无标记传感器
A novel label-free electrochemical sensor for Hg2+ based on the catalytic formation of metal nanoparticle.
机构信息
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China; Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350002, China.
出版信息
Biosens Bioelectron. 2014 Sep 15;59:1-5. doi: 10.1016/j.bios.2014.02.074. Epub 2014 Mar 6.
A novel label-free electrochemical sensor has been developed for the highly sensitive and selective detection of Hg(2+). Hg(2+) were first captured on the modified electrode surface through the specific thymine-Hg(2+)-thymine (T-Hg(2+)-T) coordination and then through the catalytic HAuCl4/NH2OH reaction for the formation of gold nanoparticles (AuNPs) as signal reporter. The formed AuNPs could be directly detected by stripping voltammetry. By the introduction of graphene to accelerate electron transfer and amplify the electrochemical signal, a detection limit as low as 0.06 nM could be obtained for Hg(2+). Compared with the traditional metal nanoparticles (NPs)-based method, this sensor avoids the labeling of the DNA probe with NP tags, only one unlabeled T-rich DNA sequence was needed, which greatly reduced the cost and simplified the sensing procedure.
一种新型的无标记电化学传感器已被开发出来，用于高灵敏度和选择性检测 Hg(2+)。Hg(2+)首先通过特异性的胸腺嘧啶-Hg(2+)-胸腺嘧啶（T-Hg(2+)-T）配位在修饰电极表面上被捕获，然后通过催化 HAuCl4/NH2OH 反应形成金纳米粒子（AuNPs）作为信号报告物。形成的 AuNPs 可以通过溶出伏安法直接检测。通过引入石墨烯来加速电子转移并放大电化学信号，对于 Hg(2+)，可以获得低至 0.06 nM 的检测限。与传统的基于金属纳米粒子（NPs）的方法相比，该传感器避免了用 NP 标签标记 DNA 探针，只需要一个未标记的富含 T 的 DNA 序列，这大大降低了成本并简化了传感过程。
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