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1
Sodium channels from human brain RNA expressed in Xenopus oocytes. Basic electrophysiologic characteristics and their modification by diphenylhydantoin.在非洲爪蟾卵母细胞中表达的来自人类大脑RNA的钠通道。基本电生理特性及其被苯妥英修饰的情况。
J Clin Invest. 1989 May;83(5):1724-32. doi: 10.1172/JCI114073.
2
Human cardiac sodium channels expressed in Xenopus oocytes.非洲爪蟾卵母细胞中表达的人类心脏钠通道。
Am J Physiol. 1990 Mar;258(3 Pt 2):H903-6. doi: 10.1152/ajpheart.1990.258.3.H903.
3
Electrophysiological characterization of a TTX-sensitive sodium current in native Xenopus oocytes.非洲爪蟾卵母细胞中一种TTX敏感钠电流的电生理特性
Proc Biol Sci. 1992 Nov 23;250(1328):127-32. doi: 10.1098/rspb.1992.0140.
4
Protein kinase A reduces voltage-dependent Na+ current in Xenopus oocytes.蛋白激酶A降低非洲爪蟾卵母细胞中的电压依赖性钠电流。
J Neurosci. 1992 Oct;12(10):3743-52. doi: 10.1523/JNEUROSCI.12-10-03743.1992.
5
[Voltage-gated influx ion channels expressed in Xenopus oocytes after the administration of brain mRNA].[给予脑信使核糖核酸后在非洲爪蟾卵母细胞中表达的电压门控性内流离子通道]
Neirofiziologiia. 1991;23(3):344-53.
6
State-dependent block of rat Nav1.4 sodium channels expressed in xenopus oocytes by pyrazoline-type insecticides.吡唑啉类杀虫剂对非洲爪蟾卵母细胞中表达的大鼠Nav1.4钠通道的状态依赖性阻断作用。
Neurotoxicology. 2005 Jun;26(3):397-406. doi: 10.1016/j.neuro.2005.03.001.
7
The effects of volatile aromatic anesthetics on voltage-gated Na+ channels expressed in Xenopus oocytes.挥发性芳香族麻醉剂对非洲爪蟾卵母细胞中表达的电压门控性钠离子通道的影响。
Anesth Analg. 2008 Nov;107(5):1579-86. doi: 10.1213/ane.0b013e318184b966.
8
Properties of a sodium channel (Na(x)) activated by strong depolarization of Xenopus oocytes.非洲爪蟾卵母细胞强烈去极化激活的钠通道（Na(x)）的特性
J Membr Biol. 2002 Feb 1;185(3):237-47. doi: 10.1007/s00232-001-0126-x. Epub 2002 Feb 13.
9
Tetrodotoxin-sensitive voltage-dependent Na currents recorded from Xenopus oocytes injected with mammalian cardiac muscle RNA.从注射了哺乳动物心肌RNA的非洲爪蟾卵母细胞记录到的河豚毒素敏感的电压依赖性钠电流。
Brain Res. 1988 Apr;427(2):187-91. doi: 10.1016/0169-328x(88)90065-4.
10
Expression of cardiac Na channels with appropriate physiological and pharmacological properties in Xenopus oocytes.具有适当生理和药理特性的心脏钠通道在非洲爪蟾卵母细胞中的表达。
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4071-4. doi: 10.1073/pnas.88.10.4071.

引用本文的文献

1
Functional characterization of the pentapeptide QYNAD on rNav1.2 channels and its NMR structure.五肽QYNAD对rNav1.2通道的功能表征及其核磁共振结构
Pflugers Arch. 2004 Mar;447(6):895-907. doi: 10.1007/s00424-003-1194-9. Epub 2003 Dec 23.
2
Electrophysiological actions of phenytoin on N-methyl-D-aspartate receptor-mediated responses in rat hippocampus in vitro.苯妥英对体外培养的大鼠海马中N-甲基-D-天冬氨酸受体介导反应的电生理作用
Br J Pharmacol. 1995 May;115(1):67-72. doi: 10.1111/j.1476-5381.1995.tb16320.x.
3
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.非洲爪蟾卵母细胞在质膜蛋白功能表达中的应用。
J Membr Biol. 1990 Sep;117(3):201-21. doi: 10.1007/BF01868451.
4
Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23.通过聚合酶链反应直接扩增单个解剖的染色体片段：人类脑钠通道基因位于2号染色体q22-q23区域。
Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):335-9. doi: 10.1073/pnas.88.2.335.

本文引用的文献

1
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
2
Neuronal mechanisms of seizure initiation.癫痫发作起始的神经元机制。
Adv Neurol. 1980;27:169-83.
3
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.非洲爪蟾卵母细胞膜中的胆碱能和儿茶酚胺能受体。
J Physiol. 1982 Jul;328:143-70. doi: 10.1113/jphysiol.1982.sp014257.
4
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.从小鼠骨髓瘤肿瘤总RNA中纯化小鼠免疫球蛋白重链信使RNA。
Eur J Biochem. 1980 Jun;107(2):303-14. doi: 10.1111/j.1432-1033.1980.tb06030.x.
5
Mechanisms of use-dependent block of sodium channels in excitable membranes by local anesthetics.局部麻醉药对可兴奋膜中钠通道的使用依赖性阻滞机制。
Biophys J. 1984 Jul;46(1):15-27. doi: 10.1016/S0006-3495(84)83994-6.
6
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.来自人类大脑的信使核糖核酸在非洲爪蟾卵母细胞中诱导药物和电压门控通道。
Nature. 1984;308(5958):421-4. doi: 10.1038/308421a0.
7
Inhibition of voltage-sensitive sodium channels in neuroblastoma cells and synaptosomes by the anticonvulsant drugs diphenylhydantoin and carbamazepine.抗惊厥药物苯妥英和卡马西平对神经母细胞瘤细胞和突触体中电压敏感性钠通道的抑制作用。
Mol Pharmacol. 1984 Mar;25(2):228-34.
8
Characterization of the block of sodium channels by phenytoin in mouse neuroblastoma cells.苯妥英对小鼠神经母细胞瘤细胞中钠通道的阻滞作用特性
J Pharmacol Exp Ther. 1984 Feb;228(2):523-30.
9
Multiple actions of phenytoin on mouse spinal cord neurons in cell culture.苯妥英对细胞培养中的小鼠脊髓神经元的多种作用。
J Pharmacol Exp Ther. 1983 Dec;227(3):779-89.
10
Lidocaine block of cardiac sodium channels.利多卡因对心脏钠通道的阻滞作用。
J Gen Physiol. 1983 May;81(5):613-42. doi: 10.1085/jgp.81.5.613.

在非洲爪蟾卵母细胞中表达的来自人类大脑RNA的钠通道。基本电生理特性及其被苯妥英修饰的情况。

Sodium channels from human brain RNA expressed in Xenopus oocytes. Basic electrophysiologic characteristics and their modification by diphenylhydantoin.

作者信息

Tomaselli G F, Marban E, Yellen G

机构信息

Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21205.

出版信息

J Clin Invest. 1989 May;83(5):1724-32. doi: 10.1172/JCI114073.

DOI:10.1172/JCI114073
PMID:2468690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC303882/
Abstract

We describe the expression and characterization of sodium channels from human brain RNA in the Xenopus oocyte. The expressed channel, studied by whole-cell voltage clamp, reveals characteristic selectivity for sodium as the permeant ion, voltage-dependent gating, and block by nanomolar concentrations of tetrodotoxin. Such channels are not seen in control oocytes injected with solvent only. The anticonvulsant diphenylhydantoin (DPH) inhibits the expressed channel in a voltage- and use-dependent manner, much like the effect seen in primary mammalian neuronal preparations. The inhibition of the expressed human sodium channel by DPH can be described by models previously developed to explain block of Na channels by local anesthetics. The preferential block of Na channels during depolarization helps explain the selectivity of DPH for neurons involved in seizure activity.

摘要

我们描述了从人脑中提取的RNA在非洲爪蟾卵母细胞中表达的钠通道及其特性。通过全细胞膜片钳技术研究该表达通道，发现其对通透离子钠具有特征性选择性、电压依赖性门控以及被纳摩尔浓度的河豚毒素阻断。在仅注射溶剂的对照卵母细胞中未观察到此类通道。抗惊厥药苯妥英（DPH）以电压和使用依赖性方式抑制该表达通道，这与在原代哺乳动物神经元制剂中观察到的效应非常相似。DPH对所表达的人钠通道的抑制作用可用先前开发的用于解释局部麻醉药对钠通道阻断作用的模型来描述。去极化过程中钠通道的优先阻断有助于解释DPH对参与癫痫活动神经元的选择性。