Zhang Huimin, Song Yanling, Zou Yuan, Ge Yun, An Yuan, Ma Yanli, Zhu Zhi, Yang Chaoyong James
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China.
Chem Commun (Camb). 2014 May 18;50(38):4891-4. doi: 10.1039/c4cc01528b.
A photo-reactive functional labelling reagent, diazirine phosphoramidite, was designed and synthesized for easy and flexible site-specific labelling of oligonucleotides with the diazirine moiety. The new reagent allows facile photo-crosslinking of oligonucleotide with its interacting partner for a variety of applications, including tertiary structure determination, molecular interaction study and biomarker discovery.
一种光反应性功能标记试剂——重氮丙啶亚磷酰胺被设计并合成出来，用于将重氮丙啶部分轻松灵活地位点特异性标记到寡核苷酸上。这种新试剂能使寡核苷酸与其相互作用伙伴轻松进行光交联，以用于多种应用，包括三级结构测定、分子相互作用研究和生物标志物发现。
