去甲丙咪嗪对大鼠离体心脏在全心缺血期间缺血诱导的细胞外钾离子、钠离子、氢离子浓度变化及去甲肾上腺素释放的影响。

The effect of desipramine on ischemia-induced changes in extracellular K+, Na+, and H+ concentrations and noradrenaline release in the isolated rat heart during global ischemia.

作者信息

Knopf H, Theising R, Hirche H

机构信息

Department of Applied Physiology, University of Cologne, F.R.G.

出版信息

J Cardiovasc Pharmacol. 1988;12 Suppl 1:S8-14. doi: 10.1097/00005344-198806121-00003.

DOI:10.1097/00005344-198806121-00003

PMID:2468840

Abstract

Isolated Langendorff-perfused rat hearts set up to allow measurement of mechanical myocardial function (according to Bardenheuer and Schrader) were subjected to 60 min of global ischemia (gI). The hearts were perfused with a modified Krebs-Henseleit solution (KHS; control group) or KHS with desipramine (DMI group) 100 nM. Extracellular concentrations of K+, Na+, and H+ were determined using ion-selective membrane polyvinyl chloride (PVC) minielectrodes. The noradrenaline (NA) release was estimated by analyzing the NA overflow during a 10-min reperfusion period, using high pressure liquid chromatography (HPLC). Control hearts and DMI-treated hearts showed no differences in mechanical performance prior to gI. In control hearts, the onset of gI resulted in a rapid triphasic [K+]e accumulation (an increase from 4.25 +/- 0.25 to 8.76 +/- 0.37 mmol/L during the first 3.5 min, a decrease to 8.06 +/- 0.34 mmol/L until the seventh min, and a further increase to 33.0 +/- 2.42 mmol/L at 60 min), a rapid increase in [H+]e (45.9 +/- 2.3 to 1314.5 +/- 214 nmol/L during 23 min), and a slight initial increase of [Na+]e (138.8 +/- 0.4 to 141.9 +/- 0.8 mmol/L within 8 min) followed by a long-lasting decrease (119.1 +/- 4.5 mmol/L at 60 min). Treatment with DMI had only slight effects on ionic redistribution during the course of ischemia. The [K-]e and [H-]e accumulation tended to be diminished, whereas [Na-]e values were slightly higher in the course of gI compared to the control group.(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

将分离的Langendorff灌注大鼠心脏设置为可测量心肌机械功能（根据Bardenheuer和Schrader的方法），使其经历60分钟的全心缺血（gI）。心脏用改良的Krebs-Henseleit溶液（KHS；对照组）或含100 nM地昔帕明（DMI组）的KHS灌注。使用离子选择性膜聚氯乙烯（PVC）微型电极测定细胞外K⁺、Na⁺和H⁺浓度。通过使用高压液相色谱（HPLC）分析10分钟再灌注期的去甲肾上腺素（NA）溢出量来估计NA释放。在gI之前，对照心脏和DMI处理的心脏在机械性能上没有差异。在对照心脏中，gI的开始导致细胞外[K⁺]迅速出现三相积累（在最初3.5分钟内从4.25±0.25 mmol/L增加到8.76±0.37 mmol/L，到第7分钟降至8.06±0.34 mmol/L，在60分钟时进一步增加到33.0±2.42 mmol/L），细胞外[H⁺]迅速增加（在23分钟内从45.9±2.3增加到1314.5±214 nmol/L），细胞外[Na⁺]最初略有增加（在8分钟内从138.8±0.4增加到141.9±0.8 mmol/L），随后长期下降（在60分钟时为119.1±4.5 mmol/L）。DMI处理在缺血过程中对离子再分布只有轻微影响。与对照组相比，gI过程中细胞外[K⁺]和[H⁺]的积累趋于减少，而细胞外[Na⁺]值略高。（摘要截短至250字）