转录活性染色质可被DNA酶I从多头绒泡菌基因组中选择性释放。
Transcriptionally active chromatin can be selectively released by DNase I from Physarum polycephalum genome.
作者信息
Fronk J, Jerzmanowski A, Wiśniewski J, Czupryn M, Toczko K, Wilhelm M L, Wilhelm F X
机构信息
Institute of Biochemistry, Warsaw University, Poland.
出版信息
Acta Biochim Pol. 1988;35(3):191-8.
In a simple eukaryote Physarum polycephalum about 13% of the genome is transcribed into abundant cytoplasmic RNA as shown by S1 nuclease digestion of DNA-RNA hybrids. Mild digestion of isolated Physarum nuclei with DNase I liberates a fraction of chromatin 3.5-fold enriched in sequences hybridizing by Physarum poly(A)+ RNA. This fraction is similarly enriched in histone H4 and actin genes known to be actively transcribed in Physarum. High content (about 45%) of actively transcribed sequences in DNase-I-released fraction of Physarum chromatin makes it particularly well suited for studying the structural basis of transcriptional activation in eukaryotes.
在一种简单的真核生物多头绒泡菌中，约13%的基因组被转录为丰富的细胞质RNA，这是通过对DNA-RNA杂交体进行S1核酸酶消化所显示的。用DNase I对分离的多头绒泡菌细胞核进行温和消化，可释放出一部分染色质，该染色质中与多头绒泡菌多聚腺苷酸（poly(A)+）RNA杂交的序列富集了3.5倍。该部分在已知在多头绒泡菌中活跃转录的组蛋白H4和肌动蛋白基因中同样富集。多头绒泡菌染色质的DNase-I释放部分中活跃转录序列的高含量（约45%）使其特别适合用于研究真核生物转录激活的结构基础。
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