Bauer J, Ganter U, Gerok W
Medizinische Universitätsklinik Freiburg, West Germany.
Adv Exp Med Biol. 1988;240:425-32. doi: 10.1007/978-1-4613-1057-0_52.
Monocytes were prepared from human blood and allowed to differentiate into macrophages in vitro. Synthesis and secretion of the potent proteinase inhibitor alpha 2-macroglobulin is strongly induced during maturation of monocytes into macrophages establishing alpha 2-macroglobulin as a differentiation marker protein. When the monocytes were incubated with endotoxin (S. typhi) in a concentration as low as 100 ng/ml, induction of alpha 2-macroglobulin synthesis was completely abolished indicating impaired monocyte/macrophage differentiation by lipopolysaccharides (LPS). In contrast to alpha 2-macroglobulin, synthesis of alpha 1-proteinase inhibitor in mononuclear phagocytes was stimulated by endotoxin.
单核细胞取自人血，在体外分化为巨噬细胞。在单核细胞成熟为巨噬细胞的过程中，强力蛋白酶抑制剂α2-巨球蛋白的合成与分泌被强烈诱导，确立了α2-巨球蛋白作为一种分化标记蛋白。当单核细胞与低至100 ng/ml浓度的内毒素（伤寒沙门氏菌）一起孵育时，α2-巨球蛋白合成的诱导被完全消除，表明脂多糖（LPS）损害了单核细胞/巨噬细胞的分化。与α2-巨球蛋白相反，单核吞噬细胞中α1-蛋白酶抑制剂的合成受到内毒素的刺激。
