Amini Sabrieh, Fathi Fardin, Mobalegi Jafar, Sofimajidpour Heshmatollah, Ghadimi Tayyeb
Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Anat Cell Biol. 2014 Mar;47(1):1-11. doi: 10.5115/acb.2014.47.1.1. Epub 2014 Mar 13.
Uncontrolled self-renewal plays a direct function in the progression of different types of carcinomas. The same molecular pathway that manages self-renewal in normal stem cells also seems to manage cancer stem cells. Here, we examine the expressions of self-renewal regulatory factors Oct4, Nanog, Sox2, nucleostemin, Zfx, Esrrb, Tcl1, Tbx3, and Dppa4 in tissue samples of colon, prostate, and bladder carcinomas as well as cancer cell lines HT-29, Caco-2, HT-1376, LNCaP, and HepG2. We used reverse transcriptase polymerase chain reaction to examine expressions of the above mentioned regulatory factors in cancer cell lines HT-29, Caco-2, HT-1376, LNCaP, and HepG2 and in 20 tumor tissue samples. Total RNA was isolated by the ISOGEN method. RNA integrity was checked by agarose gel electrophoresis and spectrophotometry. Expressions of Oct4 and nucleostemin at the protein level were determined by immunocytochemistry. A significant relationship was found between tumor grade and self-renewal gene expression. Expressions of stem cell specific marker genes were detected in all examined cancer cell lines, in 40% to 100% of bladder cancer samples, and in 60% to 100% of colon and prostate cancer samples. Oct4 expressed in 100% of tumor tissue samples. Our data show that stem cell markers Oct4, Nanog, Sox2, nucleostemin, Bmi, Zfx, Esrrb, Tcl1, Tbx3, and Dppa4 significantly express in cancer cell lines and cancer tissues. Hence, these markers might be useful as potential tumor markers in the diagnosis and/or prognosis of tumors.
不受控制的自我更新在不同类型癌症的进展中起直接作用。在正常干细胞中调控自我更新的相同分子途径似乎也调控着癌症干细胞。在此，我们检测了自我更新调节因子Oct4、Nanog、Sox2、核干细胞因子、Zfx、Esrrb、Tcl1、Tbx3和Dppa4在结肠癌、前列腺癌和膀胱癌组织样本以及癌细胞系HT-29、Caco-2、HT-1376、LNCaP和HepG2中的表达。我们使用逆转录聚合酶链反应检测上述调节因子在癌细胞系HT-29、Caco-2、HT-1376、LNCaP和HepG2以及20个肿瘤组织样本中的表达。通过ISOGEN法分离总RNA。通过琼脂糖凝胶电泳和分光光度法检查RNA完整性。通过免疫细胞化学测定Oct4和核干细胞因子在蛋白水平的表达。发现肿瘤分级与自我更新基因表达之间存在显著相关性。在所有检测的癌细胞系、40%至100%的膀胱癌样本以及60%至100%的结肠癌和前列腺癌样本中检测到干细胞特异性标记基因的表达。Oct4在100%的肿瘤组织样本中表达。我们的数据表明干细胞标记物Oct4、Nanog、Sox2、核干细胞因子、Bmi、Zfx、Esrrb、Tcl1、Tbx3和Dppa4在癌细胞系和癌组织中显著表达。因此，这些标记物可能作为潜在的肿瘤标记物用于肿瘤的诊断和/或预后评估。
