Wall R S, Gyi T J
Open University, Oxford Research Unit, United Kingdom.
Anal Biochem. 1988 Nov 15;175(1):298-9. doi: 10.1016/0003-2697(88)90392-2.
A method is described here for Alcian blue staining of proteoglycans in polyacrylamide gels; this is illustrated using extracts obtained from bovine corneal stroma. Other available methods for visualization of proteoglycans can produce nonspecific staining and frequently a high background in the gel; with a "critical electrolyte concentration" approach, specific staining against a clear background can be obtained.
本文描述了一种在聚丙烯酰胺凝胶中对蛋白聚糖进行阿尔新蓝染色的方法；使用从牛角膜基质中提取的提取物对此进行了说明。其他现有的蛋白聚糖可视化方法可能会产生非特异性染色，并且凝胶中经常出现高背景；采用“临界电解质浓度”方法，可以在清晰的背景下获得特异性染色。
