Nakai Michiko, Ozawa Manabu, Maedomari Naoki, Noguchi Junko, Kaneko Hiroyuki, Ito Junya, Onishi Akira, Kashiwazaki Naomi, Kikuchi Kazuhiro
Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan.
J Reprod Dev. 2014;60(3):256-9. doi: 10.1262/jrd.2013-100. Epub 2014 Apr 1.
In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At 10 h after ICSI or IVF, we selected only zygotes, and each of the zygotes/embryos was evaluated for cleavage every 24 h until 168 h. We then observed a delay in the 1st and 2nd cleavages of h-ICSI embryos and also in blastocoele formation by w-ICSI embryos in comparison with IVF embryos. The rate of blastocyst formation and the quality of blastocysts in both ICSI groups were inferior to those in the IVF group. In conclusion, the delay in cleavage of porcine ICSI embryos shows poorer embryonic development.
在猪中，卵胞浆内单精子注射（ICSI）后的胚胎发育能力低于体外受精（IVF）后的胚胎发育能力。我们以全精子（w-ICSI）或仅精子头部（h-ICSI）进行ICSI，并以IVF作为对照，评估了ICSI后直至囊胚形成过程中细胞分裂的时间对胚胎发育的影响。在ICSI或IVF后10小时，我们仅选择受精卵，并每24小时评估每个受精卵/胚胎的卵裂情况，直至168小时。然后我们观察到，与IVF胚胎相比，h-ICSI胚胎的第一次和第二次卵裂以及w-ICSI胚胎的囊胚腔形成均延迟。两个ICSI组的囊胚形成率和囊胚质量均低于IVF组。总之，猪ICSI胚胎的卵裂延迟表明其胚胎发育较差。
