Liao Hua, Chen Rongchang, Guan Wenda, Yang Zifeng, Liu Wenkuan, Zhou Rong, Zhong Nanshan
State Key Laboratory of Respiratory Diseases, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
Email:
Zhonghua Jie He He Hu Xi Za Zhi. 2014 Jan;37(1):21-3.
To compare the rhinovirus detection rate and the viral load in nasal samples versus lower airway samples from patients with COPD, and therefore to provide evidence for sampling selection for detection of rhinovirus.
Nasal swab and induced sputum were collected from patients with COPD during acute exacerbation and the stable period. Rhinovirus was detected by real-time fluorescent quantitative polymerase chain reaction. The difference in detection rates of rhinovirus between acute exacerbation and stable COPD was compared. The detection rates and the viral load from nasal samples versus induced sputum were also compared.
A total of 639 paired nasal swab and induced sputum specimens were collected between September 2009 and January 2013, including 114 paired specimens from COPD patients with acute exacerbations 114 paired specimens from stable COPD (matching with the stable one), and 411 paired specimens from stable COPD patients. For the 114 paired samples from stable and acute COPD patients, there was a higher detection rate in samples [nasal swab 13.2% (15/114) , induced sputum 21.9% (25/114) ] from patients with acute exacerbation, compared those with stable disease [nasal swab 3.5% (4/114), P < 0.05; and induced sputum 5.3% (6/114) P < 0.01, respectively]. Forty-two (6.6%) of the 639 nasal swab specimens were positive for rhinovirus, while 58(9.1%) of the 639 induced sputum specimens were positive for rhinovirus (P < 0.05).For the 27 paired rhinovirus positive specimens, the mean viral load of rhinovirus in induced sputum was (62.1 ± 9.5) × 10⁸ copies/L, significantly higher than that of the nasal swab (3.4 ± 0.5) × 10⁸ copies/L, P < 0.05.
For patients with COPD, induced sputum specimens may be more suitable for rhinovirus detection compared to nasal swabs, and the load of rhinovirus was higher in the lower airways than in the upper airways.
比较慢性阻塞性肺疾病（COPD）患者鼻腔样本与下呼吸道样本中鼻病毒的检出率及病毒载量，从而为鼻病毒检测的采样选择提供依据。
收集COPD患者急性加重期和稳定期的鼻拭子和诱导痰。采用实时荧光定量聚合酶链反应检测鼻病毒。比较急性加重期和稳定期COPD患者鼻病毒检出率的差异。同时比较鼻腔样本与诱导痰的检出率及病毒载量。
2009年9月至2013年1月共收集639对鼻拭子和诱导痰标本，其中包括114对COPD急性加重期患者的标本、114对稳定期COPD患者（与稳定期配对）的标本以及411对稳定期COPD患者的标本。对于114对稳定期和急性加重期COPD患者的样本，急性加重期患者样本[鼻拭子13.2%（15/114），诱导痰21.9%（25/114）]的检出率高于稳定期患者[鼻拭子3.5%（4/114），P<0.05；诱导痰5.3%（6/114），P<0.01]。639份鼻拭子标本中有42份（6.6%）鼻病毒检测呈阳性，而639份诱导痰标本中有58份（9.1%）鼻病毒检测呈阳性（P<0.05）。对于27对鼻病毒阳性标本，诱导痰中鼻病毒的平均病毒载量为（62.1±9.5）×10⁸拷贝/L，显著高于鼻拭子的（3.4±0.5）×10⁸拷贝/L，P<0.05。
对于COPD患者，与鼻拭子相比，诱导痰标本可能更适合用于鼻病毒检测，且鼻病毒在下呼吸道的载量高于上呼吸道。
