Poot M, Schmitt H, Seyschab H, Koehler J, Chen U, Kaempf U, Kubbies M, Schindler D, Rabinovitch P S, Hoehn H
Department of Human Genetics, University of Wuerzburg, Federal Republic of Germany.
Cytometry. 1989 Mar;10(2):222-6. doi: 10.1002/cyto.990100215.
Most techniques of flow cytometric cell cycle analysis are not capable of distinguishing the number of rounds of DNA synthesis that a cell has undergone since the start of an experiment. Continuous labeling with 5-bromodeoxyuridine (BrdUrd) offers such a potential. We illustrate here that the bivariate analysis of non-BrdUrd-quenched ethidium bromide vs. BrdUrd-quenched Hoechst 33258 fluorescence offers a high degree of resolution that enhances the analytical power of the technique, and that this approach can be applied to the analysis of a broad range of human and murine primary cells and established cell lines.
大多数流式细胞术细胞周期分析技术无法区分自实验开始以来细胞经历的DNA合成轮数。用5-溴脱氧尿苷（BrdUrd）进行连续标记提供了这样一种潜力。我们在此表明，对未用BrdUrd淬灭的溴化乙锭与用BrdUrd淬灭的Hoechst 33258荧光进行双变量分析，可提供高度分辨率，增强了该技术的分析能力，并且这种方法可应用于广泛的人类和小鼠原代细胞以及已建立的细胞系的分析。
