Alpha fetoprotein and albumin gene transcripts are detected in distinct cell populations of the brain and kidney of the developing rat.

We report the cellular localization of alpha-fetoprotein (AFP) and albumin (ALB) gene transcripts in rat kidney and brain as detected by in situ hybridization on tissue sections with [35S]-labelled alpha-fetoprotein and albumin cDNA probes. Both types of mRNA were present in distinct cell populations of the developing kidney and brain. In the kidney, both gene transcripts were distributed over all developing tubular cells in the 20-day-old fetus. During the first 3 weeks of life, a gradual decrease in the expression of AFP and ALB mRNA was apparent, the rate of decrease being greater on proximal tubules than on the other tubular cells. From the 4th week onwards, a weak signal for both mRNAs persisted in the majority of the tubular cells. In the brain, all neuronal cells expressed both genes. Transcript cellular distribution was mainly cytoplasmic during fetal and early postnatal life and became predominantly nuclear at 3, 4 and 5 weeks, suggesting that posttranscriptional mechanisms are involved in the control of AFP and ALB gene expression at these stages. In the adult brain no significant signal was recorded thereafter. Coexpression of AFP and ALB transcripts by specific cell types, together with their gradual disappearance concomitant with postnatal organ maturation, suggests a possible role for these proteins in terminal differentiation processes of tubular and neuronal cells.