Hallam S E, Malpartida F, Hopwood D A
John Innes Institute, Norwich, U.K.
Gene. 1988 Dec 30;74(2):305-20. doi: 10.1016/0378-1119(88)90165-5.
The BamHI fragment containing the actIII gene, from the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2), was sequenced. The derived amino acid sequence for the actIII gene shows homology to known oxidoreductases and the actIII product is believed to be responsible for catalysing a beta-keto reductive step during assembly of the Act polyketide chain. High resolution transcript mapping identified the transcription start point at 33 nucleotides upstream of the putative translation start codon. The transcript ends in a large invertedly repeated sequence. In vivo promoter-probe studies suggest that efficient transcription of the actIII gene requires the product of the actII gene.
对来自天蓝色链霉菌A3(2)的放线紫红素（Act）生物合成基因簇中包含actIII基因的BamHI片段进行了测序。actIII基因推导的氨基酸序列与已知的氧化还原酶具有同源性，并且认为actIII产物负责在Act聚酮链组装过程中催化β-酮还原步骤。高分辨率转录图谱确定转录起始点位于推定翻译起始密码子上游33个核苷酸处。转录本在一个大的反向重复序列处结束。体内启动子探针研究表明，actIII基因的有效转录需要actII基因的产物。
