Wang Tao, Zhou Chaowei, Yuan Dengyue, Lin Fangjun, Chen Hu, Wu Hongwei, Wei Rongbin, Xin Zhiming, Liu Ju, Gao Yundi, Li Zhiqiong
Department of Aquaculture, Sichuan Agricultural University, 46# Xinkang Road, Ya'an, 625014, China.
Fish Physiol Biochem. 2014 Oct;40(5):1407-15. doi: 10.1007/s10695-014-9935-6. Epub 2014 Apr 3.
Corticotropin-releasing hormone (CRH) is a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. For a better understanding of the structure and function of the CRH gene and to study its effect on feeding regulation in cyprinid fish, the cDNA of the CRH gene from the brain of Schizothorax prenanti was cloned and sequenced. The full-length CRH cDNA consisted of 1,046 bp with an open reading frame of 489 bp encoding a protein of 162 amino acids. Real-time quantitative PCR analyses revealed that CRH was widely expressed in central and peripheral tissues. In particular, high expression level of CRH was detected in brain. Furthermore, CRH mRNA expression was examined in different brain regions, especially high in hypothalamus. In addition, there was no significant change in CRH mRNA expression in fed group compared with the fasted group in the S. prenanti hypothalamus during short-term fasting. However, CRH gene expression presented significant decrease in the hypothalamus in fasted group compared with the fed group (P < 0.05) on day 7; thereafter, re-feeding could lead to a significant increase in CRH mRNA expression in fasted group on day 9. The results suggest that the CRH may play a critical role in feeding regulation in S. prenanti.
促肾上腺皮质激素释放激素（CRH）是应激反应中内分泌、自主神经、行为和免疫反应的一种强效介质。为了更好地了解CRH基因的结构和功能，并研究其对鲤科鱼类摄食调节的影响，克隆并测序了齐口裂腹鱼脑中CRH基因的cDNA。CRH cDNA全长1046 bp，开放阅读框为489 bp，编码一个由162个氨基酸组成的蛋白质。实时定量PCR分析表明，CRH在中枢和外周组织中广泛表达。特别是，在脑中检测到CRH的高表达水平。此外，还检测了CRH mRNA在不同脑区的表达，尤其是在下丘脑中表达较高。另外，在短期禁食期间，齐口裂腹鱼下丘脑的CRH mRNA表达在喂食组和禁食组之间没有显著变化。然而，在第7天，禁食组下丘脑的CRH基因表达与喂食组相比显著下降（P < 0.05）；此后，重新喂食可导致禁食组在第9天的CRH mRNA表达显著增加。结果表明，CRH可能在齐口裂腹鱼的摄食调节中起关键作用。
