Bujdoso R, Young P, Harkiss G D, McConnell I
Department of Veterinary Pathology, University of Edinburgh, U.K.
Immunology. 1989 Apr;66(4):559-64.
Antigen-specific sheep T-cell lines have been generated in vitro from peripheral blood mononuclear cells (PBMC). PBMC prepared from antigen-primed animals were cultured in the presence of ovalbumin (OVA) or purified protein derivative (PPD). After 5 days of culture, activated antigen-specific cells were expanded by further culture in the presence of recombinant human interleukin-2 (IL-2). Cell lines generated after two cycles of antigen stimulation followed by expansion with IL-2 show a proliferative response to antigen only in the presence of autologous antigen-presenting cells (APC) and recognize only the antigen used in the original stimulation. An OVA-specific cell line was found to be capable of recognizing a synthetic peptide corresponding to amino acid residues 323-338 of OVA. The cell lines also responded by proliferation in an allogeneic mixed leucocyte reaction (MLR). Cell-surface phenotyping shows that the cell lines comprise both CD4- and CD8-positive cells.
已从外周血单核细胞（PBMC）体外生成抗原特异性绵羊T细胞系。从经抗原致敏动物制备的PBMC在卵清蛋白（OVA）或纯化蛋白衍生物（PPD）存在的情况下进行培养。培养5天后，通过在重组人白细胞介素-2（IL-2）存在下进一步培养来扩增活化的抗原特异性细胞。经过两轮抗原刺激后用IL-2扩增产生的细胞系仅在自体抗原呈递细胞（APC）存在的情况下对抗原表现出增殖反应，并且仅识别原始刺激中使用的抗原。发现一种OVA特异性细胞系能够识别与OVA氨基酸残基323 - 338相对应的合成肽。这些细胞系在同种异体混合淋巴细胞反应（MLR）中也通过增殖作出反应。细胞表面表型分析表明，这些细胞系包含CD4阳性和CD8阳性细胞。
