Sousa M, Azevedo C
Department of Cell Biology, University of Oporto, Porto, Portugal.
Histochemistry. 1989;90(5):353-7. doi: 10.1007/BF00508312.
Starfish oocytes were examined before fertilization, immediately after insemination, and during the cortical reaction by means of acid phosphatase and ruthenium red ultrastructural histochemistry. Oocyte cortical granules are composed of a lamellar body and a surrounding matrix which is subdivided into dense and light portions. In unfertilized oocytes cortical granules are not stained by ruthenium red but show a weak acid phosphatase activity in the light portion of the granule matrix. Immediately after the adhesion of the spermatozoon to the oocyte jelly coat, the light matrix portion of cortical granules appears stained by ruthenium red and shows a strong acid phosphatase activity. During the cortical reaction, cortical granules are released into the perivitelline space and the lamellar body, surrounded by the stained matrix, fuses with the fertilization envelope. Our data suggest that membrane permeability changes and enzyme activation occur in the egg when the spermatozoon binds to the oocyte jelly coat.
通过酸性磷酸酶和钌红超微结构组织化学方法，对海星卵母细胞在受精前、授精后即刻以及皮层反应期间进行了检查。卵母细胞皮层颗粒由一个板层体和周围的基质组成，该基质又分为致密和浅色部分。在未受精的卵母细胞中，皮层颗粒不被钌红染色，但在颗粒基质的浅色部分显示出微弱的酸性磷酸酶活性。精子附着于卵母细胞卵黄膜后即刻，皮层颗粒的浅色基质部分被钌红染色，并显示出强烈的酸性磷酸酶活性。在皮层反应期间，皮层颗粒释放到卵周隙中，被染色基质包围的板层体与受精膜融合。我们的数据表明，当精子与卵母细胞卵黄膜结合时，卵中会发生膜通透性变化和酶激活。
