Seyoum Berhanu, Asmamaw Dawit, Iwnetu Rahel, Yamuah Lawrence K, Wassie Liya, Abebe Markos, Amanuel Yimtubezenash W, Aseffa Abraham
Ethiop Med J. 2014 Jan;Suppl 1:7-14.
Tuberculous lymphadenitis (TBLN) is a common form of extra pulmonary tuberculosis where lymph nodes particularly from cervical, axillary and inguinal sites are mostly involved, however, its diagnosis poses a major challenge in resource limited settings.
To identify the etiological species of Mycobacteria responsible for TBLN in Derra area, a rural district in Ethiopia, where the status of TBLN is unknown.
A total of 153 patients who were clinically suspected for TBLN, between the periods of August 2004 and February 2005 were included in the study. Fine needle aspirates (FNA) were collected and processed from 145 participants and further analyzed using Ziehl Neelsen staining, culture, cytology and polymerase chain reaction (PCR) using sets of primers, targeting the IS6110 insertion sequence and the pncA gene allelic variation at position 169.
Out of the 145 FNA samples, 66 (45.5%) demonstrated growth on Lowenstein-Jensen (LJ) medium and 115 (79.3%) cases were confirmed as TBLN by a combination of results from acid fast bacilli (AFB) smear examination, culture, cytology and PCR. From the 145 clinically suspected TBLN cases, 108 (75%) were identified by PCR at complex level of which 107/108 (99.1%) were positive for M. tuberculosis and 1/108 (0.9%) was positive for M. bovis using pncA primers.
The study indicates that M. tuberculosis is the major cause of tuberculous lymphadenitis in Dera area.
结核性淋巴结炎（TBLN）是肺外结核病的一种常见形式，主要累及颈部、腋窝和腹股沟部位的淋巴结，然而，在资源有限的环境中，其诊断面临重大挑战。
确定埃塞俄比亚农村地区德拉地区导致TBLN的分枝杆菌病原种类，该地区TBLN的状况未知。
2004年8月至2005年2月期间，共有153例临床疑似TBLN的患者纳入研究。从145名参与者中收集细针穿刺抽吸物（FNA）并进行处理，进一步使用萋-尼染色、培养、细胞学检查以及针对IS6110插入序列和第169位pncA基因等位变异的引物进行聚合酶链反应（PCR）分析。
在145份FNA样本中，66份（45.5%）在罗-琴（LJ）培养基上生长，115例（79.3%）通过抗酸杆菌（AFB）涂片检查、培养、细胞学检查和PCR结果综合确诊为TBLN。在145例临床疑似TBLN病例中，108例（75%）通过PCR在复合水平上得以鉴定，其中107/108（99.1%）使用pncA引物对结核分枝杆菌呈阳性，1/108（0.9%）对牛分枝杆菌呈阳性。
该研究表明结核分枝杆菌是德拉地区结核性淋巴结炎的主要病因。
