Peng Tangjian, Zhong Shuiping, Chen Chaoran, Peng Cheng, Jiang Chengying
Wei Sheng Wu Xue Bao. 2013 Dec 4;53(12):1318-25.
The work aimed to isolate and culture the acidophilic and moderately thermophilic microorganisms for leaching the sulfide ore.
We enriched and incubated iron- or sulfur-oxidizing strains from muddy water of acuric hot spring utilizing ferrous irons or elemental sulfur as substrates. Then, we identified the strains by their morphological, physiological, biochemical properties and phylogenetic positions, and estimated their bioleaching potential according to their oxidation rate of pyrite.
Two acidophilic, aerobic and facultative heterotrophic bacterial strains, Costa C and Costa E, were isolated from the samples of sulfuric hot springs of Costa Rica. Cells of the two strains were gram-positive, spore-forming and rod-shaped [(0.4 - 0.6) microm x (2.5 - 4.0) microm and (0.4 - 0.7) microm x (2.4 - 4.9) microm, respectively]. Strain Costa C grew at a temperature range of 30 degrees C - 55 degrees C and a pH range of 1.2 - 5.0, optimally at 50 degrees C and 2.8. Strain Costa E grew at a temperature range of 30 degrees C - 55 degrees C and at a pH range of 1.4 - 5.0, optimally at 40 degrees C and 2.8. Two strains could autotrophically grow on inorganic substrates such as ferrous irons, element sulfur and K2 S4 O6 and also could utilize organic substrates like yeast extract for heterotrophic growth. Phylogenetic analysis based on 16S rRNA gene sequences alignment demonstrated that the highest similarity between strain Costa C, Costa E and other species of the genus Sulfobacillus was above 99%.
Based on morphological, physiological and biochemical analysis, Costa C and Costa E can be affiliated to the genus Sulfobacillus, for which the names Sulfobacillus sp. strain Costa C and Sulfobacillus sp. strain Costa E were proposed. Both strains could oxidize pyrite, and the oxidation rates arrived 63.0 mg/L x d and 56.8 mg/L x d, respectively.
本研究旨在分离和培养嗜酸及中度嗜热微生物用于硫化矿的浸出。
利用亚铁离子或单质硫作为底物，从酸性温泉的泥水中富集并培养铁氧化或硫氧化菌株。然后，通过菌株的形态、生理、生化特性及系统发育位置对其进行鉴定，并根据其对黄铁矿的氧化速率评估其生物浸出潜力。
从哥斯达黎加硫酸温泉样品中分离出两株嗜酸、好氧兼性异养细菌菌株，Costa C和Costa E。两株菌的细胞均为革兰氏阳性、产芽孢且呈杆状[分别为(0.4 - 0.6)微米×(2.5 - 4.0)微米和(0.4 - 0.7)微米×(2.4 - 4.9)微米]。Costa C菌株在30℃ - 55℃温度范围和1.2 - 5.0 pH范围内生长，最适生长温度为50℃，最适pH为2.8。Costa E菌株在30℃ - 55℃温度范围和1.4 - 5.0 pH范围内生长，最适生长温度为40℃，最适pH为2.8。两株菌均可在亚铁离子、单质硫和连四硫酸钾等无机底物上自养生长，也可利用酵母提取物等有机底物进行异养生长。基于16S rRNA基因序列比对的系统发育分析表明，Costa C菌株、Costa E菌株与硫杆菌属其他物种之间的最高相似度高于99%。
基于形态、生理和生化分析，Costa C和Costa E可归属于硫杆菌属，为此提出硫杆菌属菌株Costa C和硫杆菌属菌株Costa E的名称。两株菌均可氧化黄铁矿，氧化速率分别达到63.0毫克/升·天和56.8毫克/升·天。
