Sudareva Natalia, Popova Helen, Saprykina Natalia, Bronnikov Sergei
Institute of Macromolecular Compounds of the Russian Academy of Sciences , St. Petersburg , Russian Federation.
J Microencapsul. 2014;31(4):333-43. doi: 10.3109/02652048.2013.858788. Epub 2014 Apr 3.
The calcium carbonate (CaCO3) cores being templates for model proteins encapsulation were obtained for developing oral drug delivery systems. The influence of the characteristics of the core formation (the time, the temperature, the stirring intensity, the ultrasound treatment and drying conditions) on the size and morphology of the carbonate cores was studied. The core size was shown to decrease with increasing the stirring time and stirring intensity. Statistical analysis of the scanning electron microscopy images of the carbonate cores allowed finding a correlation between their mean diameter and the parameters of the core formation. The regularities of proteins loading into porous CaCO3 cores were determined, and different loading methods were compared quantitatively. The co-precipitation method gives cores with the proteins load about five times as much as the adsorption method. The influence of protein properties and the ionic environment of protein molecules on the loading parameters were shown.
为开发口服给药系统，制备了用作模型蛋白包封模板的碳酸钙（CaCO₃）核。研究了核形成特性（时间、温度、搅拌强度、超声处理和干燥条件）对碳酸盐核尺寸和形态的影响。结果表明，核尺寸随搅拌时间和搅拌强度的增加而减小。对碳酸盐核的扫描电子显微镜图像进行统计分析，发现其平均直径与核形成参数之间存在相关性。确定了蛋白质负载到多孔CaCO₃核中的规律，并对不同的负载方法进行了定量比较。共沉淀法得到的核中蛋白质负载量约为吸附法的五倍。研究了蛋白质性质和蛋白质分子离子环境对负载参数的影响。
