MacDonald S M, Schleimer R P, Kagey-Sobotka A, Gillis S, Lichtenstein L M
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21239.
J Immunol. 1989 May 15;142(10):3527-32.
Human rIL-3 induces histamine release from some human basophils, with cells from atopics responding to a greater extent than non-atopic donors. The dose response curves were highly variable. IL-3 was active on purified basophils and the release process was slower and required more calcium than anti-IgE. Removal of surface IgE from basophils rendered them unresponsive to IL-3. The response could be restored by passive sensitization of basophils with IgE+, IgE known to bind histamine-releasing factors, and not IgE-, IgE unreactive with histamine-releasing factors. Thus, IL-3 uncovers IgE heterogeneity. IL-3 does not, however, directly interact with IgE+. Rather, passive sensitization with IgE+ or stimulation of basophils with low concentrations of several secretagogues renders the cells sensitive to IL-3. IL-3 may well play a pro-inflammatory role by potentiating the effects of IgE+ or various secretagogues.
人重组白细胞介素-3可诱导一些人嗜碱性粒细胞释放组胺，与非特应性供体的细胞相比，特应性个体的细胞反应程度更高。剂量反应曲线高度可变。白细胞介素-3对纯化的嗜碱性粒细胞有活性，且释放过程比抗IgE慢，需要更多的钙。去除嗜碱性粒细胞表面的IgE会使其对白细胞介素-3无反应。通过用已知能结合组胺释放因子的IgE +（而非与组胺释放因子无反应的IgE -）对嗜碱性粒细胞进行被动致敏，可恢复反应。因此，白细胞介素-3揭示了IgE的异质性。然而，白细胞介素-3并不直接与IgE +相互作用。相反，用IgE +进行被动致敏或用低浓度的几种促分泌剂刺激嗜碱性粒细胞会使细胞对白细胞介素-3敏感。白细胞介素-3很可能通过增强IgE +或各种促分泌剂的作用而发挥促炎作用。
