Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic; Department of Phototrophic Microorganisms, Institute of Microbiology CAS, Opatovický mlýn, Třeboň, Czech Republic.
FEMS Microbiol Ecol. 2014 Aug;89(2):303-15. doi: 10.1111/1574-6941.12299. Epub 2014 Mar 7.
Here, we report an effect of short acclimation to a wide span of temperatures on photosynthetic electron transfer, lipid and fatty acid composition in the snow alga Chlamydomonas cf. nivalis. The growth and oxygen evolution capacity were low at 2 °C yet progressively enhanced at 10 °C and were significantly higher at temperatures from 5 to 15 °C in comparison with the mesophilic control Chlamydomonas reinhardtii. In search of the molecular mechanisms responsible for the adaptation of photosynthesis to low temperatures, we have found unprecedented high rates of QA to QB electron transfer. The thermodynamics of the process revealed the existence of an increased structural flexibility that we explain with the amino acid changes in the D1 protein combined with the physico-chemical characteristics of the thylakoid membrane composed of > 80% negatively charged phosphatidylglycerol.
在这里，我们报告了短时间适应宽温度范围对雪藻 Chlamydomonas cf. nivalis 光合作用电子传递、脂质和脂肪酸组成的影响。在 2°C 时，生长和氧气释放能力较低，但在 10°C 时逐渐增强，与中温对照品 Chlamydomonas reinhardtii 相比，在 5 至 15°C 的温度下显著更高。为了寻找光合作用适应低温的分子机制，我们发现了前所未有的 QA 到 QB 电子传递的高速率。该过程的热力学揭示了结构灵活性的增加，我们用 D1 蛋白中的氨基酸变化来解释，结合由>80%带负电荷的磷脂酰甘油组成的类囊体膜的物理化学特性。
