Chakraborti S, Nag T C, Das D, Sanyal Chatterjee T, De S K
Department of Zoology, A.P.C. Roy Government College, Siliguri 734010, West Bengal, India.
Department of Anatomy, All India Institute of Medical Sciences, New Delhi 110029, India.
Tissue Cell. 2014 Jun;46(3):165-9. doi: 10.1016/j.tice.2014.03.001. Epub 2014 Mar 18.
We have examined cytokeratin distribution and their nature in toe pads of the Himalayan tree-frog Philautus annandalii. Toe pads are expanded tips of digits and show modifications of their ventral epidermis for adhesion. The toe pad epidermal cells, being organized into 3-4 rows, possess keratin bundles, especially in surface nanostructures that are involved in adhesion. Immunohistochemical localization using a pan-cytokeratin antibody revealed that cytokeratin immunoreactivity is the strongest in the mid- to basal cell rows of the epidermis, which parallels our previous ultrastructural observation of dense keratin bundles present in this part of the epidermis. The remainder of the epidermis (i.e., the superficial cell layer) showed little immunoreactivity. Immunoblot analysis revealed that toe-pads possessed keratins prominently in the molecular mass of 50 kDa. Possible presence of keratin 5 in toe pad epidermis has been correlated with its usual distribution pattern in mammalian epidermis.
我们研究了喜马拉雅树蛙（Philautus annandalii）趾垫中细胞角蛋白的分布及其性质。趾垫是指端膨大的部分，其腹侧表皮有用于粘附的特化结构。趾垫表皮细胞排列成3 - 4排，含有角蛋白束，尤其是在参与粘附的表面纳米结构中。使用泛细胞角蛋白抗体进行免疫组织化学定位显示，细胞角蛋白免疫反应性在表皮的中基细胞排中最强，这与我们之前对该表皮部分存在密集角蛋白束的超微结构观察结果一致。表皮的其余部分（即表层细胞层）显示出很少的免疫反应性。免疫印迹分析表明，趾垫中突出存在分子量为50 kDa的角蛋白。趾垫表皮中可能存在的角蛋白5与其在哺乳动物表皮中的常见分布模式相关。
