Syrjänen Leo, Luukkaala Tiina, Leppilampi Mari, Kallioinen Matti, Pastorekova Silvia, Pastorek Jaromir, Waheed Abdul, Sly William S, Parkkila Seppo, Karttunen Tuomo
Institute of Biomedical Technology and School of Medicine, University of Tampere, Tampere, Finland.
APMIS. 2014 Sep;122(9):880-9. doi: 10.1111/apm.12251. Epub 2014 Apr 3.
Purpose of the study was to evaluate the presence of hypoxia-inducible, tumour-associated carbonic anhydrases IX and XII in normal skin and a series of cutaneous tumours. Human tumour samples were taken during surgical operations performed on 245 patients and were immunohistochemically stained. A histological score value was calculated for statistical analyses which were performed using SPSS for Windows, versions 17.0 and 20.0. In normal skin, the highest expression of CA IX was detected in hair follicles, sebaceous glands, and basal parts of epidermis. CA XII was detected in all epithelial components of skin. Both CA IX and CA XII expression levels were significantly different in epidermal, appendigeal, and melanocytic tumour categories. Both CA IX and XII showed the most intense immunostaining in epidermal tumours, whereas virtually all melanocytic tumours were devoid of CA IX and XII immunostaining. In premalignant lesions, CA IX expression significantly increased when the tumours progressed to more severe dysplasia forms. Both CA IX and XII are highly expressed in different epithelial components of skin. They are also highly expressed in epidermal tumours, in which CA IX expression levels also correlate with the dysplasia grade. Interestingly, both isozymes are absent in melanocytic tumours.
本研究的目的是评估缺氧诱导的肿瘤相关碳酸酐酶IX和XII在正常皮肤及一系列皮肤肿瘤中的存在情况。在对245例患者进行的外科手术过程中采集人类肿瘤样本，并进行免疫组织化学染色。计算组织学评分值以进行统计分析，统计分析使用Windows版SPSS 17.0和20.0版本进行。在正常皮肤中，CA IX在毛囊、皮脂腺和表皮基部检测到最高表达。CA XII在皮肤的所有上皮成分中均有检测到。CA IX和CA XII的表达水平在表皮、附属器和黑素细胞肿瘤类别中均有显著差异。CA IX和XII在表皮肿瘤中显示出最强的免疫染色，而几乎所有黑素细胞肿瘤均无CA IX和XII免疫染色。在癌前病变中，当肿瘤进展为更严重的发育异常形式时，CA IX表达显著增加。CA IX和XII在皮肤的不同上皮成分中均高表达。它们在表皮肿瘤中也高表达，其中CA IX表达水平也与发育异常分级相关。有趣的是，两种同工酶在黑素细胞肿瘤中均不存在。
