Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
Nutrition. 2014 May;30(5):563-8. doi: 10.1016/j.nut.2013.10.012. Epub 2013 Oct 30.
Acute lymphocytic leukemia (ALL) and its subsequent treatment may provoke increased oxidative stress. The aim of this study was to investigate the antioxidant status of children and adolescents who had received ALL therapy, and to test the hypothesis that selenium (Se) inadequacy is correlated with reduced defenses against oxidative stress in this population.
This case-control study involved 24 patients between ages 5 and 13 y who had been treated successfully for ALL (ALL group) and 60 children of similar age and socioeconomic background with no clinical history of leukemia (control group). Dietary intake of Se was evaluated by the 24-h recall method, and the concentrations of Se in plasma, erythrocytes, and urine determined. Antioxidant status was assessed by analysis of the oxidative stress markers, namely, superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), α-tocopherol, and 8-oxo-deoxyguanosine (8-oxo-dG).
There were no between-group differences with respect to plasma (P = 0.122), erythrocyte (P = 0.202), urinary (P = 0.608), or dietary (P = 0.757) levels of Se. GPx activity was significantly (P < 0.001) reduced in the ALL group compared with the control group, whereas SOD activity and MDA concentrations were similar. The concentrations of α-tocopherol and 8-oxo-dG were significantly increased in the ALL group compared with the control group (P < 0.001 and P = 0.031, respectively).
All participants were Se inadequate, but such inadequacy was not correlated with reduced defenses against oxidative stress. However, individuals of the ALL group were with increased oxidative stress compared with the control group, possibly due to previous disease and to intensive polychemotherapy.
急性淋巴细胞白血病（ALL）及其后续治疗可能会引发氧化应激增加。本研究旨在调查接受 ALL 治疗的儿童和青少年的抗氧化状态，并验证硒（Se）不足与该人群对抗氧化应激能力降低相关的假设。
本病例对照研究纳入了 24 名年龄在 5 至 13 岁之间、成功接受 ALL 治疗的患者（ALL 组）和 60 名年龄和社会经济背景相似、无白血病临床病史的儿童（对照组）。通过 24 小时回忆法评估 Se 的膳食摄入量，并测定血浆、红细胞和尿液中的 Se 浓度。通过分析氧化应激标志物，即超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、丙二醛（MDA）、α-生育酚和 8-氧代-脱氧鸟苷（8-oxo-dG），评估抗氧化状态。
两组间血浆（P = 0.122）、红细胞（P = 0.202）、尿（P = 0.608）或膳食（P = 0.757）中的 Se 水平均无差异。与对照组相比，ALL 组的 GPx 活性显著降低（P < 0.001），而 SOD 活性和 MDA 浓度相似。与对照组相比，ALL 组的 α-生育酚和 8-oxo-dG 浓度显著升高（P < 0.001 和 P = 0.031）。
所有参与者均存在 Se 不足，但这种不足与抗氧化应激能力降低无关。然而，与对照组相比，ALL 组的个体存在更高的氧化应激，可能是由于先前的疾病和强化化疗所致。
