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[采用纸电泳和醋酸纤维素电泳评估香草扁桃酸的排泄情况]

[Evaluation of the excretion of vanilmandelic acid using paper and cellulose acetate electrophoresis].

作者信息

Svistunova O I, Lipitskaia I Ia, Kiseleva Z M, Titov V N

出版信息

Lab Delo. 1989(3):61-4.

PMID:2469847
Abstract

The authors compare the results of vanillylmandelic acid (VMA) excretion measurements by high-voltage electrophoresis on paper and by microelectrophoresis on cellulose acetate. Transverse scanning of electrophoregrams has been used for quantitative evaluation of the results of microelectrophoregrams on cellulose acetate. The results of VMA excretion measurements by the two methods have been in good correlation: r = 0.93, n = 15, y = 0.17 + 0.78x, p less than 0.01. Electrophoresis on cellulose acetate is three times less labor consuming and can be performed with Soviet equipment and maintenance medium. The excretion intensity can be assessed visually by reference values of VMA concentration.

摘要

作者比较了用纸上高压电泳法和醋酸纤维素薄膜微电泳法测量香草扁桃酸(VMA)排泄量的结果。已采用电泳图谱横向扫描对醋酸纤维素薄膜微电泳结果进行定量评估。两种方法测量VMA排泄量的结果具有良好的相关性:r = 0.93,n = 15,y = 0.17 + 0.78x,p<0.01。醋酸纤维素薄膜电泳的工作量减少了三分之二,并且可以使用苏联的设备和维护介质来进行。排泄强度可通过VMA浓度的参考值进行目测评估。

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