用于定量检测神经内分泌肿瘤标志物的高效液相色谱-质谱法：香草扁桃酸、高香草酸和5-羟吲哚乙酸。

HPLC-mass spectrometry method for quantitative detection of neuroendocrine tumor markers: vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid.

作者信息

Lionetto Luana, Lostia Alfonso M, Stigliano Antonio, Cardelli Patrizia, Simmaco Maurizio

机构信息

UO Diagnostica Molecolare Avanzata, Azienda Ospedaliera Sant'Andrea, Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", II Facoltà di Medicina e Chirurgia, Università La Sapienza, Rome, Italy.

出版信息

Clin Chim Acta. 2008 Dec;398(1-2):53-6. doi: 10.1016/j.cca.2008.08.003. Epub 2008 Aug 8.

DOI:10.1016/j.cca.2008.08.003

PMID:18760269

Abstract

BACKGROUND

The urinary excretion of vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) can be increased in the presence of neuroblastic and carcinoid tumors. The former is characterized by defective catecholamine metabolism which results in high urinary levels of VMA and HVA. The latter shows an altered metabolism of tryptophan and an increased synthesis of serotonin, producing high 5-HIAA urinary concentrations.

METHODS

We describe an HPLC-tandem mass spectrometric method for the simultaneous quantification of VMA, HVA and 5-HIAA in human urine. The chromatographic separation is performed on a reversed-phase C18 column. Instrumental analysis is performed on a Q-Trap 2000 triple quadrupole/ion trap mass spectrometer.

RESULTS

The method is fast and does not require sample pre-treatment. Multiple calibration curve exhibited consistent linearity and reproducibility. Linear responses were observed in the concentration range 0-50 mg/l for each analyte. Limits of detection were 0.001 mg/l for VMA, 0.015 mg/l for 5-HIAA and 0.050 mg/l for HVA with a signal-to-noise ratio of 3. Limits of quantification were 0.005 mg/l for VMA, 0.050 mg/l for 5-HIAA and 0.1 mg/l for HVA with a signal-to-noise ratio of 10.

CONCLUSIONS

This method can be proposed as a tool for neuroendocrine tumor markers detection.

摘要

背景

在存在神经母细胞瘤和类癌肿瘤的情况下，香草扁桃酸（VMA）、高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）的尿排泄量可能会增加。前者的特征是儿茶酚胺代谢缺陷，导致尿中VMA和HVA水平升高。后者表现为色氨酸代谢改变和血清素合成增加，导致尿中5-HIAA浓度升高。

方法

我们描述了一种用于同时定量人尿中VMA、HVA和5-HIAA的高效液相色谱-串联质谱法。色谱分离在反相C18柱上进行。仪器分析在Q-Trap 2000三重四极杆/离子阱质谱仪上进行。

结果

该方法快速，无需样品预处理。多条校准曲线表现出一致的线性和重现性。每种分析物在0-50 mg/l的浓度范围内均观察到线性响应。VMA的检测限为0.001 mg/l，5-HIAA为0.015 mg/l，HVA为0.050 mg/l，信噪比为3。VMA的定量限为0.005 mg/l，5-HIAA为0.050 mg/l，HVA为0.1 mg/l，信噪比为10。