Mattos Douglas da Cruz, Cardoso Leonardo Demier, Fosse Paulo José, Radael Marcella Costa, Fosse Filho João Carlos, Manhães João Vitor de Azevedo, de Andrade Dalcio Ricardo, Vidal Manuel Vazquez
Universidade Estadual do Norte Fluminense Darcy Ribeiro,Departamento de Produção Animal,Laboratório de Zootecnia e Nutrição Animal,CEP,Campos dos Goytacazes,RJ,Brazil.
Zygote. 2015 Jun;23(3):460-6. doi: 10.1017/S0967199414000069. Epub 2014 Apr 3.
The aim of this study was to describe the embryonic and larval development of discus fish (Symphysodon aequifasciatus), and to determine the time required, hours post fertilization (hpf), for the emergence of various structures. To describe embryonic and larval stages, observations were made at regular periods under an optical microscope and images were taken with a digital camera attached to a microscope. The average temperature reached in the experimental tank was 27.9°C. Important facts in embryonic and larval development are described, such as the closure of the blastopore, which occurred at 31.5 hpf; a period of eruption, which occurred at 58.5 hpf; and a mouth opening, which occurred at 90.5 hpf; and larvae that exhibited oriented swimming and eating exogenous food at 136.5 hpf.
本研究的目的是描述七彩神仙鱼(盘丽鱼)的胚胎和幼体发育,并确定受精后不同结构出现所需的时间(以受精后小时数,即hpf表示)。为了描述胚胎和幼体阶段,在光学显微镜下定期进行观察,并用连接到显微镜的数码相机拍照。实验水箱中的平均温度达到27.9°C。文中描述了胚胎和幼体发育中的重要事件,如胚孔闭合发生在受精后31.5小时;出膜期发生在受精后58.5小时;开口发生在受精后90.5小时;幼体在受精后136.5小时开始定向游动并摄食外源食物。