利用小鼠血管外植体在小型化三维胶原凝胶中进行血管生成
Angiogenesis Utilizing Murine Vascular Explants in Miniaturized 3-Dimensional Collagen Gels.
作者信息
Reed May J, Damodarasamy Mamatha, Vernon Robert B
机构信息
Department of Medicine, University of Washington, Harborview Medical Center, 325 Ninth Ave., Seattle, WA 98104, USA.
Hope Heart Matrix Biology Program, Benaroya Research Institute at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101-2795, USA.
出版信息
Open Circ Vasc J. 2011 May 9;4:12-17. doi: 10.2174/1877382601104010012.
Abstract
Models of angiogenesis are used to study blood vessel morphogenesis and the effects of compounds that influence vascular growth. Herein, we describe techniques to induce angiogenesis-like sprouting from explants of mouse aortae and microvessels cultured in 3-dimensional gels of native type I collagen. The gels are supported by rings of nylon mesh that are sized to fit in 96-well culture plates. This mechanically-supported, miniaturized, 3-dimensional culture system requires only small quantities of cells and reagents and facilitates handling, staining, and imaging by conventional and confocal microscopy.
摘要
血管生成模型用于研究血管形态发生以及影响血管生长的化合物的作用。在此，我们描述了从小鼠主动脉外植体和培养在天然I型胶原蛋白三维凝胶中的微血管诱导血管生成样芽生的技术。凝胶由尼龙网环支撑，其尺寸适合96孔培养板。这种机械支撑的小型化三维培养系统仅需要少量细胞和试剂，并便于通过传统显微镜和共聚焦显微镜进行处理、染色和成像。
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