通过DNA转染使小鼠成纤维细胞定向分化为脂肪细胞

Commitment of mouse fibroblasts to adipocyte differentiation by DNA transfection.

作者信息

Chen S, Teicher L C, Kazim D, Pollack R E, Wise L S

机构信息

Department of Biological Sciences, Columbia University, New York, NY 10027.

出版信息

Science. 1989 May 5;244(4904):582-5. doi: 10.1126/science.2470149.

DOI:10.1126/science.2470149

PMID:2470149

Abstract

Cells of the mouse cell line 3T3-F442A can be induced by various hormones to differentiate into adipocytes, whereas cells of 3T3-C2, a subclone of 3T3, cannot. However, transfection of DNA from uninduced 3T3-F422A cells into 3T3-C2 cells permits recovery of 3T3-C2 transfectants that differentiate into adipocytes in the presence of insulin. DNA isolated from human fat tissue, when transfected into 3T3-C2 mouse cells, also gives rise to mouse transfectants that are induced to differentiate into adipocytes by the addition of insulin. Apparently, transfection of a trans-regulatory gene (or genes) from 3T3-F442A or human fat cells into 3T3-C2 cells is sufficient to commit 3T3-C2 cells to adipocyte differentiation.

摘要

小鼠细胞系3T3-F442A的细胞可被多种激素诱导分化为脂肪细胞，而3T3的亚克隆3T3-C2细胞则不能。然而，将未诱导的3T3-F422A细胞的DNA转染到3T3-C2细胞中，可使3T3-C2转染子在胰岛素存在的情况下分化为脂肪细胞。从人脂肪组织分离的DNA转染到3T3-C2小鼠细胞中时，也会产生在添加胰岛素后被诱导分化为脂肪细胞的小鼠转染子。显然，将3T3-F442A或人脂肪细胞中的反式调节基因转染到3T3-C2细胞中足以使3T3-C2细胞进行脂肪细胞分化。