Li Hui, Zhang Mingjie, Zhang Xiaomei, Li Heng, Shi Jinsong, Xu Zhenghong
Sheng Wu Gong Cheng Xue Bao. 2013 Nov;29(11):1687-91.
In order to improve transformation efficiency of dehydroepiandrosterone (DHEA) into 3beta,7alpha,15alpha-trihydroxy-5-androsten-17-one (7alpha,15alpha-diOH-DHEA) by Gibberella intermedia CA3-1, we investigated the strains breeding and their conversion process optimization. G. intermedia CA3-1 strains were treated with 0.12 mg/mL 1-methyl-3-nitro-1-nitroso-guanidin (NTG) for 30 min and chosen by 350 micromol/L minimum inhibitory concentration ketoconazole resistance marker. The high production strain named M-10 with a good genetic stability was selected and the product molar yield achieved to 70.2%, which was 20% higher than that of original strain. Under the improved conversion process with the DHEA concentration of 5 g/L, the product molar yield of the mutant M-10 reached 75.6%, which was improved by 31.3% than that of original strain.
为提高中间型赤霉菌CA3-1将脱氢表雄酮（DHEA）转化为3β,7α,15α-三羟基-5-雄烯-17-酮（7α,15α-二羟基-DHEA）的效率，我们研究了菌株选育及其转化过程优化。中间型赤霉菌CA3-1菌株用0.12 mg/mL的1-甲基-3-硝基-1-亚硝基胍（NTG）处理30分钟，并通过350 μmol/L的最低抑菌浓度酮康唑抗性标记进行筛选。筛选出高产菌株M-10，其遗传稳定性良好，产物摩尔产率达到70.2%，比原始菌株高20%。在DHEA浓度为5 g/L的改进转化过程下，突变体M-10的产物摩尔产率达到75.6%，比原始菌株提高了31.3%。
