Environ Technol. 2014 May-Jun;35(9-12):1470-7. doi: 10.1080/09593330.2013.871045.
The biodegradation potential of Burkholderia vietnamiensis G4 (B. vietnamiensis G4) was evaluated under encapsulation in comparison with direct exposure to trichloroethylene (TCE) (0.1, 0.5, 1 and 5 mg/L) and toluene (10 and 50 mg/L), maintaining aerobic conditions. B. vietnamiensis G4 was encapsulated in polyethylene glycol (PEG) polymer. Under suspended conditions, the degradation rate decreased as the initial TCE concentration increased, even with a higher amount of substrate available. However, the encapsulated systems were less suppressed, presumably by mitigated toxicity, and completely removed TCE with 50 mg/L of toluene. The transformation yield (Ty) was as high as 0.427 mg-TCE/mg-toluene for the encapsulated cultures and 0.1007 mg-TCE/mg-toluene for the suspended cultures. The Ty value for the encapsulated cultures was one to two orders higher than what has been reported in the literature. The higher Ty values in the encapsulated cultures compared with those from suspended cultures showed that the PEG encapsulation provided more a favourable environment for efficient substrate use.
在有氧条件下，将伯克霍尔德氏菌 G4（B. vietnamiensis G4）进行包埋处理，并与直接暴露于三氯乙烯（TCE）（0.1、0.5、1 和 5mg/L）和甲苯（10 和 50mg/L）进行比较，评估其生物降解潜力。B. vietnamiensis G4 被包埋在聚乙二醇（PEG）聚合物中。在悬浮条件下，随着初始 TCE 浓度的增加，降解速率降低，即使有更多的底物可用。然而，包埋系统受到的抑制较小，可能是由于毒性减轻，并且用 50mg/L 的甲苯完全去除了 TCE。转化产率（Ty）对于包埋培养物高达 0.427mg-TCE/mg-甲苯，对于悬浮培养物高达 0.1007mg-TCE/mg-甲苯。与文献报道相比，包埋培养物的 Ty 值高出 1 到 2 个数量级。与悬浮培养物相比，包埋培养物中更高的 Ty 值表明 PEG 包埋为有效利用底物提供了更有利的环境。
